CAJ | 학술논문

得到脂肪酶固定化的最佳条件及固定脂肪酶对生物柴油的转化率.利用单因素试验和正交试验确定脂肪酶固定化的最佳条件,通过气相色谱-质谱联用仪分析合成生物柴油的主要成分.结果:脂肪酶固定化的最佳条件:缓冲液pH 7.5、载体/酶液比为25 mg/mL、固定化温度应为40℃,其固定化酶活力为68.25 U/g,利用固定脂肪酶催化合成生物柴油的转化率高达75%.结果表明:固定化酶比游离酶具有更高的催化效率,对高温和pH具有更高的耐受性.固定化脂肪酶催化效率高、操作稳定性较强,具有较好的工业化生产潜力.
득도지방매고정화적최가조건급고정지방매대생물시유적전화솔.이용단인소시험화정교시험학정지방매고정화적최가조건,통과기상색보-질보련용의분석합성생물시유적주요성분.결과:지방매고정화적최가조건:완충액pH 7.5、재체/매액비위25 mg/mL、고정화온도응위40℃,기고정화매활력위68.25 U/g,이용고정지방매최화합성생물시유적전화솔고체75%.결과표명:고정화매비유리매구유경고적최화효솔,대고온화pH구유경고적내수성.고정화지방매최화효솔고、조작은정성교강,구유교호적공업화생산잠력.
It was to get the best conditions of lipase immobilization and the conversion rate of biodiesel production. The optimum conditions of lipase immobilization were obtained by single factor experiment and orthogonal test. The main components of the biodiesel were analyzed by the gas chromatograph-mass spectrometer. The optimum conditions of lipase immobilization were obtained that was the pH of buffer was 7.5 , the ration of vector : enzyme solution was 25 mg : 1 mL, the immobilized temperature was 40 ℃. The immobilized enzyme activity was 68.25 U/g. With immobilized lipase the conversion rate of biodiesel production was up to 75%. The result showed that the immobilized lipase had higher catalytic efficiency and its tolerance was more improved to high-temperature and pH than the free enzyme. The immobilized lipase had a potential for industrial production because of its high catalytic efficiency and strong operational stability.