CAJ | 학술논문

比较经优化条件提取的番石榴叶总黄酮粗提物(样品A)和经AB-8大孔树脂纯化的番石榴叶总黄酮精提物(样品B)的体外生物学活性.通过清除DPPH自由基、羟自由基、还原力检测样品的抗氧化能力及研究对α-葡萄糖苷酶的抑制作用来比较样品A、B的体外生物学活性. 样品A和样品B的黄酮含量分别为7.8%、13.28%, 对DPPH自由基的清除率分别为87.61%、97.22%,对羟自由基的清除率分别为62.32%、80.45%,样品B的还原力远大于样品A;样品A和样品B对α-葡萄糖苷酶的抑制率分别为99.17%、99.81%,远大于阿卡波糖.样品A和样品B都有一定的抗氧化能力和对α-葡萄糖苷酶有一定的抑制作用,总体上样品B高于样品A,可能与其中的总黄酮含量有关.
비교경우화조건제취적번석류협총황동조제물(양품A)화경AB-8대공수지순화적번석류협총황동정제물(양품B)적체외생물학활성.통과청제DPPH자유기、간자유기、환원력검측양품적항양화능력급연구대α-포도당감매적억제작용래비교양품A、B적체외생물학활성. 양품A화양품B적황동함량분별위7.8%、13.28%, 대DPPH자유기적청제솔분별위87.61%、97.22%,대간자유기적청제솔분별위62.32%、80.45%,양품B적환원력원대우양품A;양품A화양품B대α-포도당감매적억제솔분별위99.17%、99.81%,원대우아잡파당.양품A화양품B도유일정적항양화능력화대α-포도당감매유일정적억제작용,총체상양품B고우양품A,가능여기중적총황동함량유관.
Compared the vitro bioactivity of guava leaves flavonoids crude extract by the optimal extraction conditions (sample A) and guava leaf flavonoids fine extract purified by AB-8 macroporous resin (sample B). Studied on antioxidation of samples by the DPPH radical scavenging , hydroxyl radical scavenging , reducing power andα-glucosidase inhibitory to comapre the vitro bioactivity between sample A and sample B. Flavonoids content of the sample A and sample B were 7.8%and 13.28%, with DPPH free radical scavenging rates of 87.61%and 97.22%, with hydroxyl radical scavenging rate of 62.32%and 80.45%and withα-glucosidase inhibitory rates of 99.17%and 99.81%,which are much larger than acarbose. And restoring force of sample B is much larger than the sample A. Sample A and sample B has a certain amount of antioxidant activity and α-glucosidase inhibitory effect, which may have a certain relationship with flavonoids, as a whole, sample B slightly higher than the sample.