食品研究与开发
食品研究與開髮
식품연구여개발
Food Research and Development
2015年
22期
154-158
,共5页
黄筱萍%黄国昌%刘兰%熊大维%张婷
黃篠萍%黃國昌%劉蘭%熊大維%張婷
황소평%황국창%류란%웅대유%장정
2-苯乙醇%酿酒酵母%优化%生物转化
2-苯乙醇%釀酒酵母%優化%生物轉化
2-분을순%양주효모%우화%생물전화
2-phenylethanol%Saccharomyces cerevisiae%optimization%bioconversion
以L-苯丙氨酸为底物,利用酿酒酵母转化合成2-苯乙醇,对影响酿酒细胞转化的因素进行考察.确定酵母菌转化的菌龄,通过单因素和正交试验,优化转化培养基组成和培养条件.优化后的培养基组成:葡萄糖120 g/L,酵母粉5 g/L,L-苯丙氨酸8 g/L,MgSO4 0.2 g/L,KH2PO4 0.05 mol/L,K2HPO4 0.05 mol/L,28℃200 r/min培养转化24 h,2-苯乙醇产率达4.37 g/L,比优化前提高30.1%.
以L-苯丙氨痠為底物,利用釀酒酵母轉化閤成2-苯乙醇,對影響釀酒細胞轉化的因素進行攷察.確定酵母菌轉化的菌齡,通過單因素和正交試驗,優化轉化培養基組成和培養條件.優化後的培養基組成:葡萄糖120 g/L,酵母粉5 g/L,L-苯丙氨痠8 g/L,MgSO4 0.2 g/L,KH2PO4 0.05 mol/L,K2HPO4 0.05 mol/L,28℃200 r/min培養轉化24 h,2-苯乙醇產率達4.37 g/L,比優化前提高30.1%.
이L-분병안산위저물,이용양주효모전화합성2-분을순,대영향양주세포전화적인소진행고찰.학정효모균전화적균령,통과단인소화정교시험,우화전화배양기조성화배양조건.우화후적배양기조성:포도당120 g/L,효모분5 g/L,L-분병안산8 g/L,MgSO4 0.2 g/L,KH2PO4 0.05 mol/L,K2HPO4 0.05 mol/L,28℃200 r/min배양전화24 h,2-분을순산솔체4.37 g/L,비우화전제고30.1%.
The bioconversion conditions for the production of 2-phenylethanol with Saccharomyces cerevisiae SH003 were studied. Culture time of seed liquid was determined. The experiments of single factor and orthogonal design were carried out to optimize the compositions of bioconversion and cultivation conditions for 2-phenylethanol production. The optimized medium contains 120 g/L glucose, 5 g/L yeast extract, 8 g/L L-phenylalanine,0.2 g/L MgSO4, 0.05 mol/L KH2PO4 and 0.05 mol/L K2HPO4 . Incubated at 28℃and 200 r/min for 24 h, the yield of 2-phenylethanol reached up to 4.37 g/L, increased by 30.1%compared with the original condition.
