中医临床研究
中醫臨床研究
중의림상연구
Clinical Journal of Chinese Medicine
2015年
33期
131-133
,共3页
李挺%丛超%王星际%张超%孙雷
李挺%叢超%王星際%張超%孫雷
리정%총초%왕성제%장초%손뢰
皮动蛋白%TSPAN-1%Arg%结肠肿瘤%免疫组织化学法
皮動蛋白%TSPAN-1%Arg%結腸腫瘤%免疫組織化學法
피동단백%TSPAN-1%Arg%결장종류%면역조직화학법
Skin proteins%TSPAN-1%Arg%Colon cancer%Immunohistochemistry
目的:研究皮动蛋白基因和TSPAN-1及Arg在结直肠癌组织中的表达,分析其与临床病理因素的关系.方法:采用免疫组化ELIVISION二步法,检测TSPAN-1和CORTACTIN基因及Arg蛋白在80例结直肠癌组织,13例结肠腺瘤组织及27例正常黏膜组织中的表达.结果:TSPAN-1 蛋白在正常黏膜、结肠腺瘤、结肠癌中阳性表达率分别为 7.4%、23.0%、90.0%,组间差异具有统计学意义(P<0.01);皮动蛋白在正常黏膜、结肠腺瘤、结肠癌中阳性表达率分别为 52.0%、77.0%、97.5%,组间差异具有统计学意义(P<0.01),Arg 蛋白在正常黏膜、结肠腺瘤、结肠癌中阳性表达率分别为 11.1%、30.8%、91.2%,组间差异具有统计学意义(P<0.01);皮动蛋白和TSPAN-1及Arg的表达与结肠癌的淋巴结转移、TNM分期、五年生存率、组织学分级及浸润深度有关(P<0.01);皮动蛋白和TSPAN-1及Arg在结肠癌组织中的表达强度呈正相关(r=0.397, P=0.0013)(r=0.397, P=0.0013),三者表达的一致率高达88.8%(71/80)及90.0%(72/80).结论:皮动蛋白基因的高表达与肿瘤细胞的侵袭性转移性有关,可以被用作判断结直肠癌预后的一个标志.皮动蛋白与TSPAN-1基因的高表达与结肠癌的侵袭和发展有关,二者在结肠癌的细胞增殖、浸润和转移中有协同作用,联合检测二者的表达对于进一步理解结肠癌的生物学行为和判断预后有一定价值.皮动蛋白与TSPAN-1可能作为结直肠癌检测的肿瘤标记物和治疗的分子靶点.Arg的检测对皮动蛋白与TSPAN-1基因的联合检测有协同辅助作用,三者的检测更具有准确性.
目的:研究皮動蛋白基因和TSPAN-1及Arg在結直腸癌組織中的錶達,分析其與臨床病理因素的關繫.方法:採用免疫組化ELIVISION二步法,檢測TSPAN-1和CORTACTIN基因及Arg蛋白在80例結直腸癌組織,13例結腸腺瘤組織及27例正常黏膜組織中的錶達.結果:TSPAN-1 蛋白在正常黏膜、結腸腺瘤、結腸癌中暘性錶達率分彆為 7.4%、23.0%、90.0%,組間差異具有統計學意義(P<0.01);皮動蛋白在正常黏膜、結腸腺瘤、結腸癌中暘性錶達率分彆為 52.0%、77.0%、97.5%,組間差異具有統計學意義(P<0.01),Arg 蛋白在正常黏膜、結腸腺瘤、結腸癌中暘性錶達率分彆為 11.1%、30.8%、91.2%,組間差異具有統計學意義(P<0.01);皮動蛋白和TSPAN-1及Arg的錶達與結腸癌的淋巴結轉移、TNM分期、五年生存率、組織學分級及浸潤深度有關(P<0.01);皮動蛋白和TSPAN-1及Arg在結腸癌組織中的錶達彊度呈正相關(r=0.397, P=0.0013)(r=0.397, P=0.0013),三者錶達的一緻率高達88.8%(71/80)及90.0%(72/80).結論:皮動蛋白基因的高錶達與腫瘤細胞的侵襲性轉移性有關,可以被用作判斷結直腸癌預後的一箇標誌.皮動蛋白與TSPAN-1基因的高錶達與結腸癌的侵襲和髮展有關,二者在結腸癌的細胞增殖、浸潤和轉移中有協同作用,聯閤檢測二者的錶達對于進一步理解結腸癌的生物學行為和判斷預後有一定價值.皮動蛋白與TSPAN-1可能作為結直腸癌檢測的腫瘤標記物和治療的分子靶點.Arg的檢測對皮動蛋白與TSPAN-1基因的聯閤檢測有協同輔助作用,三者的檢測更具有準確性.
목적:연구피동단백기인화TSPAN-1급Arg재결직장암조직중적표체,분석기여림상병리인소적관계.방법:채용면역조화ELIVISION이보법,검측TSPAN-1화CORTACTIN기인급Arg단백재80례결직장암조직,13례결장선류조직급27례정상점막조직중적표체.결과:TSPAN-1 단백재정상점막、결장선류、결장암중양성표체솔분별위 7.4%、23.0%、90.0%,조간차이구유통계학의의(P<0.01);피동단백재정상점막、결장선류、결장암중양성표체솔분별위 52.0%、77.0%、97.5%,조간차이구유통계학의의(P<0.01),Arg 단백재정상점막、결장선류、결장암중양성표체솔분별위 11.1%、30.8%、91.2%,조간차이구유통계학의의(P<0.01);피동단백화TSPAN-1급Arg적표체여결장암적림파결전이、TNM분기、오년생존솔、조직학분급급침윤심도유관(P<0.01);피동단백화TSPAN-1급Arg재결장암조직중적표체강도정정상관(r=0.397, P=0.0013)(r=0.397, P=0.0013),삼자표체적일치솔고체88.8%(71/80)급90.0%(72/80).결론:피동단백기인적고표체여종류세포적침습성전이성유관,가이피용작판단결직장암예후적일개표지.피동단백여TSPAN-1기인적고표체여결장암적침습화발전유관,이자재결장암적세포증식、침윤화전이중유협동작용,연합검측이자적표체대우진일보리해결장암적생물학행위화판단예후유일정개치.피동단백여TSPAN-1가능작위결직장암검측적종류표기물화치료적분자파점.Arg적검측대피동단백여TSPAN-1기인적연합검측유협동보조작용,삼자적검측경구유준학성.
Objective: To research the expression of Arg and TSPAN-1 in colorectal carcinoma tissues, and analyze its relationship with the clinical pathological factors. Methods: The expression of TSPAN-1 and CORTACTIN gene and Arg protein in 80 cases of colorectal cancer tissues, 13 cases of colorectal adenoma tissues and 27 cases of normal mucosa tissues were detected by two step method of immunohistochemical ELIVISION. TSPAN-1 protein expression in the normal mucosa, colorectal adenomas, colon cancer, the positive expression rate was 23.0%, 90.0% and 30.8%, respectively, the positive expression rate of P was 52.0%, 77.0%, 97.5%, Arg protein expression in normal mucosa, colorectal adenoma, colorectal cancer,P<0.01 protein expression and TSPAN-1, Arg and TSPAN-1, TNM, Arg, P<0.01, and,P<0.01 and. The expression level ofP=0.0013 (r=0.397) (r=0.397,P=0.0013), and the expression of the three groups were 88.8% (71/80) and 90.0% (72/80). Conclusion: The high expression of the gene is related to the invasion and metastasis of tumor cells, and it can be used as a marker for the prognosis of colorectal cancer. The high expression of gene and TSPAN-1 gene were related to the invasion and development of colon cancer. The two had a synergistic effect on cell proliferation, invasion and metastasis of colon cancer. TSPAN-1 may serve as a molecular target for tumor markers and therapeutic targets for colorectal cancer detection. The detection of Arg and TSPAN-1 gene in combination with the detection of synergistic effect, the detection of the three more accurate.
