CAJ | 학술논문

为了降低生物法制备甘露醇的成本,以假肠膜明串珠菌Leuconostoc pseudomesenteroides G123为研究对象,对培养基中的氮源、葡萄糖与果糖比例和 pH 调控过程进行了优化,提高了果糖转化率和甘露醇产量.5L 发酵罐中结果显示:采用2g/L的酵母粉作为单一氮源,葡萄糖和果糖的比例为0.35:1,初始pH值7.5,发酵过程中保持pH值不低于4.5,甘露醇产量可达57.24g/L,甘露醇对果糖的转化率为83.2%.该过程副产D-乳酸20.32g/L,其光学纯度达99.9%,具有回收价值,甘露醇与D-乳酸对糖总转化率为89.38%,有助于降低生物法制备甘露醇的成本.
위료강저생물법제비감로순적성본,이가장막명천주균Leuconostoc pseudomesenteroides G123위연구대상,대배양기중적담원、포도당여과당비례화 pH 조공과정진행료우화,제고료과당전화솔화감로순산량.5L 발효관중결과현시:채용2g/L적효모분작위단일담원,포도당화과당적비례위0.35:1,초시pH치7.5,발효과정중보지pH치불저우4.5,감로순산량가체57.24g/L,감로순대과당적전화솔위83.2%.해과정부산D-유산20.32g/L,기광학순도체99.9%,구유회수개치,감로순여D-유산대당총전화솔위89.38%,유조우강저생물법제비감로순적성본.
A new mutant strainLeuconostoc pseudomesenteroides G123 was investigated in this study. The nitrogen source,the ratio of glucose and fructose and the pH control strategy were optimized to reduce the cost of mannitol production. Batch fermentations were operated in a 5L fermentor.The results showed that 57.24g/L mannitol was produced with the mannitol yield of 83.2% when 2g/L yeast extract was chosen as the single nitrogen source,the ratio of fructose toglucose was 0.35:1,and the initial pH was controlled at 7.5 and kept constant at 4.5 in the later phase. Besides,20.32g/L D-lactic acid with optical purity of 99.9% was also produced which suggested it could be recovered as a by-product. The yield of mannitol and lactic acid to the total sugar was 89.38%. As a result,the cost was reduced efficiently.