CAJ | 학술논문

镉(Cd~(2+))是一种分布较广泛,毒性较强的一种重金属,文章利用韭菜青×IR26杂交衍生的一个重组自交系群体(Recombinant inbred lines,RIL)及构建的SSR分子遗传图谱,对控制糙米中Cd~(2+)含量的QTL进行分析,为选育籽粒中Cd~(2+)低吸收或低积累的水稻品种提供参考.结果表明,在Cd~(2+)胁迫(5 mg/kg)处理条件下,共检测到2个与糙米Cd~(2+)含量有关的QTLs,分别位于水稻第11染色体上的标记RM6288-RM6544和RM167-RM5704之间,其中qCCBR-11a对表型贡献率为11.17%,加性效应0.089;qCCBR-11b对表型变异贡献率为7.66%,加性效应0.075.相关分析显示,糙米Cd~(2+)含量与株高、每穗总粒数、每穗实粒数,结实率和千粒重等产量性状的相关性均不显著,糙米中Cd~(2+)含量是一个相对独立、由基因控制的遗传性状.
력(Cd~(2+))시일충분포교엄범,독성교강적일충중금속,문장이용구채청×IR26잡교연생적일개중조자교계군체(Recombinant inbred lines,RIL)급구건적SSR분자유전도보,대공제조미중Cd~(2+)함량적QTL진행분석,위선육자립중Cd~(2+)저흡수혹저적루적수도품충제공삼고.결과표명,재Cd~(2+)협박(5 mg/kg)처리조건하,공검측도2개여조미Cd~(2+)함량유관적QTLs,분별위우수도제11염색체상적표기RM6288-RM6544화RM167-RM5704지간,기중qCCBR-11a대표형공헌솔위11.17%,가성효응0.089;qCCBR-11b대표형변이공헌솔위7.66%,가성효응0.075.상관분석현시,조미Cd~(2+)함량여주고、매수총립수、매수실립수,결실솔화천립중등산량성상적상관성균불현저,조미중Cd~(2+)함량시일개상대독립、유기인공제적유전성상.
A population of Fs recombinant inbred lines (RILs), derived from a cross between IR26 (Oryza sativa L. spp. indica) and Jiucaiqing (japonica), were used to identify the quantitative trait loci (QTLs) for Cd~(2+) content in brown rice under 5 mg/kg Cd + stress. Two QTLs, qCCBR-lla and qCCBR-llb, associated with the Cd + content in brown rice, were detected on chromosome 11. qCCBR-lla was located at the position between markers RM6288 and RM6544, accounting for 11.17% of the phenotypic variance with an additive effect value of 0.089. qCCBR-llb at the interval between markers RM167 and RM5704 explained 7.66% of the phenotypic variance with an additive effect value of 0.075. In addition, the correlation coefficients between Cd~(2+) content of brown rice and plant height, spikelets per panicle, filled grains per panicle, seed setting rate, and 1 000-grain weight were not significant. This suggested that the Cd~(2+) content in brown rice under Cd~(2+) stress was an independent genetic trait.