CAJ | 학술논문

富含甘氨酸RNA结合蛋白(Glycine-rich RNA-bindingproteins,GRP)是植物中重要的转录后调控蛋白,在植物的生长发育及对胁迫的抗性调控等过程中起重要作用.文章从二色补血草(Limonium bicolor(Bunge)O.)cDNA文库中克隆出了富含甘氨酸RNA结合蛋白基因(LbGRP)的全长cDNA序列(GenBank登录号:GQ398238).为了研究LbGRP的抗逆功能,将其构建到酵母表达载体pYES2中,转化到酿酒酵母INVSc1菌株中,获得重组酵母INVSc1(pYES2.LbGRP),同时将转空pYES2质粒的酵母INVSc1(pYES2)作为对照.对重组酵母INVSc1(pYES2-LbGRP)和对照INVSc1(pYES2)进行NaCl、KCl、NaHCO_3、Na_2CO_3、干旱和冷冻胁迫,比较它们在不同胁迫下的存活率.结果表明,INVSc1(pYES2-LbGRP)在各种胁迫下的存活率明显高于对照INVSc1(pYES2),证明LbGRP基因具有抗NaCl、KCl、NaHCO_3、Na_2CO_3、干旱和冷冻等胁迫的能力,推测该基因参与了二色补血草的抗逆调控过程.
부함감안산RNA결합단백(Glycine-rich RNA-bindingproteins,GRP)시식물중중요적전록후조공단백,재식물적생장발육급대협박적항성조공등과정중기중요작용.문장종이색보혈초(Limonium bicolor(Bunge)O.)cDNA문고중극륭출료부함감안산RNA결합단백기인(LbGRP)적전장cDNA서렬(GenBank등록호:GQ398238).위료연구LbGRP적항역공능,장기구건도효모표체재체pYES2중,전화도양주효모INVSc1균주중,획득중조효모INVSc1(pYES2.LbGRP),동시장전공pYES2질립적효모INVSc1(pYES2)작위대조.대중조효모INVSc1(pYES2-LbGRP)화대조INVSc1(pYES2)진행NaCl、KCl、NaHCO_3、Na_2CO_3、간한화냉동협박,비교타문재불동협박하적존활솔.결과표명,INVSc1(pYES2-LbGRP)재각충협박하적존활솔명현고우대조INVSc1(pYES2),증명LbGRP기인구유항NaCl、KCl、NaHCO_3、Na_2CO_3、간한화냉동등협박적능력,추측해기인삼여료이색보혈초적항역조공과정.
Glycine-rich RNA.binding proteins(GRP) are involved in post-transcriptional regulation,and play important roles in plant growth,development and stress response.In the present study,the full-length cDNA of a GRPP gene(LbGRP,GenBank accession number:GQ398238)was cloned from a cDNA library of Limonium bicolor.To investigate the stress tolerance of LbGRP.the recombinant plasmid pYES2-LbGRP was constructed by inserting the LbGRP gene mto the yeast expression vector pYES2.The recombinant plasmid pYES2-LbGRP Was transformed into yeast Saccharomyces cerevisiae INVSc1.and the INVSc1 strain transformed with empty pYES2 was used as the control.The recombinant yeast INVSc1 harboring LbGRP(pYES2-LbGRP)and the control INVSc1 harboring empty pYES2 were treated with NaCl,KCl,NaHCO_3,Na_2CO_3,drought and frezzing,respectively,and their survial rates were compared under these stress conditions.Theresults showed that the survivaI rates of the recombinant yeast INVSc1(pYES2-LbGRP)were higher than that of the control strain under these stress conditions.indicating that the LbGRP iS tolerant to NaCl,KCl,NaHCO_3,Na_2CO_3,drought and frezzing stresses.These results suggested that LbGRP plays a rolein stress tolerance of L.bicolor.