CAJ | 학술논문

运用褶合光谱法建立了β-半乳糖苷酶的最佳褶合光谱以及该酶在最佳测试条件下的线性方程：Qj=1.906×10-2+4.946×10-3C+1.909×10-4C2,r=1.0000；对已瞬时转染β-半乳糖苷酶表达载体的COS-1细胞提取液样品中的β-半乳糖苷酶的活性进行了测定，并与吸光度值分析法相对照。结果表明：本法快速、简便、准确，在生化分析中有广泛的应用价值。
운용습합광보법건립료β-반유당감매적최가습합광보이급해매재최가측시조건하적선성방정：Qj=1.906×10-2+4.946×10-3C+1.909×10-4C2,r=1.0000；대이순시전염β-반유당감매표체재체적COS-1세포제취액양품중적β-반유당감매적활성진행료측정，병여흡광도치분석법상대조。결과표명：본법쾌속、간편、준학，재생화분석중유엄범적응용개치。
The optimum convolution spectra of β-galactosidase and the linear equation of Qj=1.906×10-2＋4.946×10-3 C＋1.909×10-4 C2,r=1.0000 under the best condition were established by the convolution spectrum method.Based on the chromogenic substrate o-nitrophenyl-β-galactoside,a method for the determination ofβ-galactosidase activity in the COS-1 extraction sample which had been transfected transiently expression vector of β-galactosidase was determined by using the convolution curve transformation technology.The convolution spectrum method was compared with the absorption method and the result was satisfactory.The method was simple,convenient,fast and valuable to the biochemical analysis.