CAJ | 학술논문

目的:探讨不同浓度氧化低密度脂蛋白(oxLDL)对细胞滋养细胞浸润能力的影响.方法:采用蛋白印迹法和RT-PCR技术检测不同浓度oxLDL(0.01,0.02,0.05,0.1 mg/mL)刺激下细胞滋养细胞中过氧化物酶体增殖物激活受体γ(PPARγ)蛋白及其mR-NA的表达:并检测相应浓度oxLDL对细胞滋养细胞能力的影响.结果:(1)不同浓度oxLDL(0.01,0.02,0.05,0.1mg/mL)刺激下细胞滋养细胞中PPAR-γ蛋白表达水平(15.0±2.0,18.4±2.8,21.3±2.4,25.8±3.0)与对照组(13.3±2.2)比较差异有统计学意义(P<0.01),各组分别比较差异亦有统计学意义P<0.01;(2)不同浓度oxLDL(0.01,0.02,0.05,0.1 mg/mL)刺激下细胞滋养细胞PPARγmRNA表达水平(14.9±3.1,17.2±4.0,19.4±3.2,21.7±3.0)与对照组(12.1±3.3)比较差异有统计学意义P<0.01,各组分别比较差异亦有统计学意义P<0.01;(3)不同浓度oxLDL(0.01,0.02,0.05,0.1mg/mL)刺激下细胞滋养细胞浸润指数(0.66±0.04,0.58±0.03,0.46±0.04,0.32±0.05),与对照组(0.69±0.05)比较差异有统计学意义P<0.01,各组分别比较差异亦有统计学意义P<0.01;(4)oxLDL浓度与细胞滋养细胞的浸润指数呈负相关,(r=-0.98,P<0.01);与细胞滋养细胞PPARγmRNA表达水平呈正相关(r=0.93,P<0.01).结论:oxLDL浓度升高可上调细胞滋养细胞中PPARγ的表达,从而影响细胞滋养细胞的浸润能力.
목적:탐토불동농도양화저밀도지단백(oxLDL)대세포자양세포침윤능력적영향.방법:채용단백인적법화RT-PCR기술검측불동농도oxLDL(0.01,0.02,0.05,0.1 mg/mL)자격하세포자양세포중과양화물매체증식물격활수체γ(PPARγ)단백급기mR-NA적표체:병검측상응농도oxLDL대세포자양세포능력적영향.결과:(1)불동농도oxLDL(0.01,0.02,0.05,0.1mg/mL)자격하세포자양세포중PPAR-γ단백표체수평(15.0±2.0,18.4±2.8,21.3±2.4,25.8±3.0)여대조조(13.3±2.2)비교차이유통계학의의(P<0.01),각조분별비교차이역유통계학의의P<0.01;(2)불동농도oxLDL(0.01,0.02,0.05,0.1 mg/mL)자격하세포자양세포PPARγmRNA표체수평(14.9±3.1,17.2±4.0,19.4±3.2,21.7±3.0)여대조조(12.1±3.3)비교차이유통계학의의P<0.01,각조분별비교차이역유통계학의의P<0.01;(3)불동농도oxLDL(0.01,0.02,0.05,0.1mg/mL)자격하세포자양세포침윤지수(0.66±0.04,0.58±0.03,0.46±0.04,0.32±0.05),여대조조(0.69±0.05)비교차이유통계학의의P<0.01,각조분별비교차이역유통계학의의P<0.01;(4)oxLDL농도여세포자양세포적침윤지수정부상관,(r=-0.98,P<0.01);여세포자양세포PPARγmRNA표체수평정정상관(r=0.93,P<0.01).결론:oxLDL농도승고가상조세포자양세포중PPARγ적표체,종이영향세포자양세포적침윤능력.