现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2009年
16期
2402-2403
,共2页
盐酸青藤碱缓释片%反相高效液相色谱法%含量测定
鹽痠青籐堿緩釋片%反相高效液相色譜法%含量測定
염산청등감완석편%반상고효액상색보법%함량측정
Sinomenine Hydrochloride Sustained-Release Tablets%HPLC%Content determination
目的:探讨建立反相高效液相色谱法(RP-HPLC)测定盐酸青藤碱缓释片中主药含量的方法.方法:色谱柱:KremasilC18,流动相为乙腈-磷酸二氢钠水溶液(85:15),检测波长265 nm,流速1 ml/min,柱温30℃,进样量20 μl.结果:盐酸青藤碱检测的线性范围为20.24～202.4 μg/ml(r=0.9992),平均回收率为100.1%,RSD=0.9%.结论:本方法简便、准确、专属性强,可用于该制剂的质量控制.
目的:探討建立反相高效液相色譜法(RP-HPLC)測定鹽痠青籐堿緩釋片中主藥含量的方法.方法:色譜柱:KremasilC18,流動相為乙腈-燐痠二氫鈉水溶液(85:15),檢測波長265 nm,流速1 ml/min,柱溫30℃,進樣量20 μl.結果:鹽痠青籐堿檢測的線性範圍為20.24～202.4 μg/ml(r=0.9992),平均迴收率為100.1%,RSD=0.9%.結論:本方法簡便、準確、專屬性彊,可用于該製劑的質量控製.
목적:탐토건립반상고효액상색보법(RP-HPLC)측정염산청등감완석편중주약함량적방법.방법:색보주:KremasilC18,류동상위을정-린산이경납수용액(85:15),검측파장265 nm,류속1 ml/min,주온30℃,진양량20 μl.결과:염산청등감검측적선성범위위20.24～202.4 μg/ml(r=0.9992),평균회수솔위100.1%,RSD=0.9%.결론:본방법간편、준학、전속성강,가용우해제제적질량공제.
Objective :To establish a method for the determination of sinomenine hydrochloride in Sinomenine Hydrochloride Sus-tained-Release Tablets. Methods:HPLC was performed on a C18 column using acetonitrile -NaH2PO4 (85:15) as the mobile phase at the flow rate of 1 ml/min. The detection wavelength was at 265 nm,The column temperature was 30 ℃ and the sample size was 20 μl. Re-sults:The linear range of sinomenine hydrochloride was 20.24～202.4 μg/ml (r=0.9992), the average recovery was 100.1%(RSD=0.9%). Conclusion:The method is simple, accurate,special and can be used for the quality control of Sinomenine Hydrechloride Sustained-Re-lease Tablets.
