CAJ | 학술논문

应用S-P免疫组织化学方法对62例膀胱移行上皮癌及6例正常膀胱粘膜组织分别检测P16蛋白、PCNA蛋白表达。结果显示62例膀胱移行细胞癌中P16的表达与膀胱癌分级间呈负相关（P＜0.005），在浸润性癌显著低于浅表性癌（P＜0.005），在复发组低于未复发组（P＜0.005）；PCNA表达与膀胱癌分级呈高度正相关（P＜0.05），浸润性癌高于浅表性癌（P＜0.01），复发组高于未复发组（P＜0.01）。P16和PCNA的表达呈显著负相关（P＜0.005）。认为检测P16、PCNA的表达对了解膀胱癌的生物学行为、膀胱癌的诊断、推测预后及治疗方式的选择有一定的临床参考价值。
응용S-P면역조직화학방법대62례방광이행상피암급6례정상방광점막조직분별검측P16단백、PCNA단백표체。결과현시62례방광이행세포암중P16적표체여방광암분급간정부상관（P＜0.005），재침윤성암현저저우천표성암（P＜0.005），재복발조저우미복발조（P＜0.005）；PCNA표체여방광암분급정고도정상관（P＜0.05），침윤성암고우천표성암（P＜0.01），복발조고우미복발조（P＜0.01）。P16화PCNA적표체정현저부상관（P＜0.005）。인위검측P16、PCNA적표체대료해방광암적생물학행위、방광암적진단、추측예후급치료방식적선택유일정적림상삼고개치。
62 patients with bladder transitional cell cancer for the firsttime were selected.The expression of P16 and PCNA was studied in consecutive tissue slides using S-P immunohistochemical technique.The results showed P16 expressed mostly in nuclear and its positive was 50.00％.PCNA expressed in nuclear and its positive percentage was 64.52％.There was negative correlation between P16 expression and the cancer’s grades，stages.Conversely PCNA expression was oppositely correlated to the cancer’s grades and stages.Statistical differences were observed for expression of P16 and PCNA between G1 and G3，superficial tumors（Tis～T1）and invasive tumors （T2～T4），the recurrence and non recurrence.These suggest that the detection of P16 and PCNA may be used to evaluate the behaviour of TCC.