CAJ | 학술논문

使用塔板理论证明存在一种使正常色谱峰产生拖尾的因素--柱出口效应。证明符合线性分配的样品组分虽然在色谱柱内存在3种不同浓度的分布形态，但在流出色谱柱后却都因柱出口效应的影响而转变成拖尾峰。在不加任何近似处理的情况下，使用塔板理论直接对不同塔板数、容量因子的色谱峰不对称性进行了计算；计算结果同样支持了柱出口效应的存在。
사용탑판이론증명존재일충사정상색보봉산생타미적인소--주출구효응。증명부합선성분배적양품조분수연재색보주내존재3충불동농도적분포형태，단재류출색보주후각도인주출구효응적영향이전변성타미봉。재불가임하근사처리적정황하，사용탑판이론직접대불동탑판수、용량인자적색보봉불대칭성진행료계산；계산결과동양지지료주출구효응적존재。
A factor-effect of column outlet could cause normal chro matographic peak to tail when the solute left the end of the column was proved b y plate theory. When the distribution coefficient was constant and independent of the amount of solute present in a plate, the distribution of solute could have three shapes in the column, but they all turned to skewed peaks when leaving the end of the column. The last plate makes the solute change their shapes to skewn ess when they left the end of the column; this factor was named the “effect of column outlet”. The asymmetry of solute inside or outside of the column was cal culated by using dircetly plate theory under the different number of plate n and partition ratio k′, A%＝（Ab－Af)/[(Ab+Af )/2 ] (Ab=back area and Af=front area of the peak, measured from a ver tival line through the base line), the A% proved to have the effect of co lumn outlet again. The effect of column outlet could be eliminated with temperat ure program in gas chromatography and elution gradient in liquid chromatography, using these methods could reduce the k′ in the last plate, therefore the n ear symmetrical peak could be obtained when use these methods.