CAJ | 학술논문

研究了灿烂甲酚蓝与脱氧核糖核酸(DNA)作用的共振光散射光谱，在pH=10.8～11．5的范围内，DNA的加入导致灿烂甲酚蓝共振光散射的增强，在347 nm处，存在一共振光散射增强峰，其强度与DNA的浓度呈线性关系，据此建立了一种测定DNA的共振光谱散射法。该方法的线性范围为80～1000 μg/L，检出限为23. 3 μg/L。
연구료찬란갑분람여탈양핵당핵산(DNA)작용적공진광산사광보，재pH=10.8～11．5적범위내，DNA적가입도치찬란갑분람공진광산사적증강，재347 nm처，존재일공진광산사증강봉，기강도여DNA적농도정선성관계，거차건립료일충측정DNA적공진광보산사법。해방법적선성범위위80～1000 μg/L，검출한위23. 3 μg/L。
The resonance light scattering (RLS) spectra of brilliant crystal blue with deoxyribonucleic acid have been studied. The RLS of brilliant crystal blue is greatly enhanced by deoxyribonucleic acid in Ph range of 11.0～11．5.There is a resonance light scattering peak at 347 nm, and the enhanced intensity of RLS at this wavelength is proportional to the concentration of deoxyri bonucleic acid. So a mehtod of RLS for the determination of deoxyribonucleic acid is establlished. The linear range of the calibration graph is 80～1000μg/L. The detection limit is 23.3 μg/L. This method is simple, rapid and has been applied to the determination of deoxyribonucleic acid in mixed samples with sati sfactory results