CAJ | 학술논문

用核磁共振氢谱和质谱法研究大鼠血浆中溴莫普林(BDP)及其代谢物。首先用固相萃取法将血液中内源性物质除去，然后经核磁共振氢谱和质谱检测大鼠血液中BDP及其代谢物。大鼠服用溴莫普林后20h时血浆中检测到两个代谢产物：脱甲基-溴莫普林葡萄糖醛酸和溴莫普林硫酸。结果证明核磁共振氢谱和质谱法可以快速、方便地用于血液中药物及其代谢产物的含量与结构检测的研究。
용핵자공진경보화질보법연구대서혈장중추막보림(BDP)급기대사물。수선용고상췌취법장혈액중내원성물질제거，연후경핵자공진경보화질보검측대서혈액중BDP급기대사물。대서복용추막보림후20h시혈장중검측도량개대사산물：탈갑기-추막보림포도당철산화추막보림류산。결과증명핵자공진경보화질보법가이쾌속、방편지용우혈액중약물급기대사산물적함량여결구검측적연구。
The methods of 1H Nuclear Magnetic Resonance (NMR) and mass spectroscopy were used in detecting the metabolites of brodimoprim (BDP) in rat plasma. The endogenic compounds in the plasma were removed with solid phase extraction (SPE) column firstly, then the mixture of metabolites was identified with 1H NMR and mass spectroscopy (MS). Two metabolites of BDP in the plasma at 20h were detected, they were demethyl-brodimorpim glucuronide and brodimoprim sulfurate. The study proved that the method of SPE coupled with NMR and MS can be applied to the analysis of metbolites in plasma quickly and conveniently.