CAJ | 학술논문

用TUNEL法对26例骨髓增生异常综合征（MDS）、11例巨幼细胞贫血（MegA）、8例阵发性血红蛋白尿（PNH）、6例Evans综合征患者和10例正常健康志愿者的骨髓单个核细胞（BMMNC）凋亡进行探讨。结果发现，50％MDS呈现凋亡过度，MegA、PNH和Evans综合征患者与对照组无显著差异。MDS患者凋亡累及粒、红、巨三系各阶段造血细胞，随病情演变，凋亡程度渐下降。提示凋亡过度为MDS无效造血机制之一，病情演变可能与异常克隆逃逸凋亡有关，抗凋亡疗法可试用于早期MDS。
용TUNEL법대26례골수증생이상종합정（MDS）、11례거유세포빈혈（MegA）、8례진발성혈홍단백뇨（PNH）、6례Evans종합정환자화10례정상건강지원자적골수단개핵세포（BMMNC）조망진행탐토。결과발현，50％MDS정현조망과도，MegA、PNH화Evans종합정환자여대조조무현저차이。MDS환자조망루급립、홍、거삼계각계단조혈세포，수병정연변，조망정도점하강。제시조망과도위MDS무효조혈궤제지일，병정연변가능여이상극륭도일조망유관，항조망요법가시용우조기MDS。
Apoptosis of bone marrow mononuclear cells（BMMNC）was demonstratedusing terminal deoxytransferase（TdT）-mediated dUTP nick end labeling（TUNEL）method in 26 myelodysplastic syndromes（MDS）patients，11 megaloblastic anemia（MegA），8 paroxysmal nocturnal hemoglobinuria（PNH）and 6 Evans syndromes patients.Apoptosis was significantly increased in MDS，no difference of apoptosis was found between MegA，PNH，Evans syndromes and normal controls.Apoptosis was seen in myeloid blasts，maturing myeloid cells，nucleated erythroid cells and megakaryocyte cells in MDS patients.As MDS progressing，apoptosis gradually decreased.These results suggest that excess apoptosis is one of factors leading to ineffective hematopoiesis in MDS.MDS clinical progression may be associated with escaping from apoptosis of myelodysplastic progenitors.Therapy of anti-apoptosis could be used in early MDS patients.