CAJ | 학술논문

利用双向电场控制毛细管电泳系统，考察了神经递质的分离。在pH 2.5的0.01 mol/L磷酸盐缓冲体系中，通过加入20%(V／V）正丙醇，改善了多巴胺和5-羟色胺的分离效果，但仍不太理想。通过施加径向电场，可进一步提高分离度。本研究不仅拓宽了径向电场调控的样品分离范围，而且为生物活性物质的痕量分析提供了参考依据。
이용쌍향전장공제모세관전영계통，고찰료신경체질적분리。재pH 2.5적0.01 mol/L린산염완충체계중，통과가입20%(V／V）정병순，개선료다파알화5-간색알적분리효과，단잉불태이상。통과시가경향전장，가진일보제고분리도。본연구불부탁관료경향전장조공적양품분리범위，이차위생물활성물질적흔량분석제공료삼고의거。
Separations of neurotransmitters such as dopamine (DA), ser otonin (5-HA), norepinephrine (NE) and epinephrine (E) were performed successf ully using a homemade electric field modulated capillary electrophoretic system, which could offer both radial and axial electric fields with only one high volt age power supply. DA and 5-HT were eluted simulaneously and could not be resolv ed in 0.01 mol/L phosphate buffer at pH 2.5. Alcohol additives, such as methanol , ethanol or 1-propanol were added to the buffer to change the solvation shell of the solutes, which changed their effective sizes and electrophoretic mobiliti es of the solutes accordingly. The optimum composition was a buffer of 20% (V ／V） 1-propanol, with resulted resolutions 0.74 (DA/5-HT), 0.56(5-HT/NE) and 0.77 (NE/E). If a positive radial voltage of 6.6 kV was applied, the resolut ions were improved to 1.48, 0.71 and 1.32, respectively.