CAJ | 학술논문

利用溶胶凝胶法,将正硅酸乙酯在HCl存在下水解形成的硅溶胶和黄曲霉毒素B_1抗体的混合液涂于玻碳电极表面, 制备非标记型电化学阻抗免疫传感器.以[Fe(CN)_6]~(3-/4-)的磷酸盐缓冲溶液为测试底液,分别研究传感器的循环伏安和交流阻抗行为.实验表明,电极因免疫反应所形成的复合物阻碍了[Fe(CN)_6]~(3-/4-)在电极表面的扩散), 其氧化还原峰电流明显减小,电子转移阻抗随黄曲霉毒素浓度增加而线性增大.当介质pH=6.5和孵育时间为20 min时,免疫前后传感器的电子转移阻抗变化值最大.在此最佳条件下,传感器电子转移阻抗对黄曲霉毒素响应的线性范围为1.0～10 μg/L;检出限为0.1 μg/L(S/N=3).此方法具有高的灵敏度和稳定性,可应用于食品中黄曲霉毒素的测定.
이용용효응효법,장정규산을지재HCl존재하수해형성적규용효화황곡매독소B_1항체적혼합액도우파탄전겁표면, 제비비표기형전화학조항면역전감기.이[Fe(CN)_6]~(3-/4-)적린산염완충용액위측시저액,분별연구전감기적순배복안화교류조항행위.실험표명,전겁인면역반응소형성적복합물조애료[Fe(CN)_6]~(3-/4-)재전겁표면적확산), 기양화환원봉전류명현감소,전자전이조항수황곡매독소농도증가이선성증대.당개질pH=6.5화부육시간위20 min시,면역전후전감기적전자전이조항변화치최대.재차최가조건하,전감기전자전이조항대황곡매독소향응적선성범위위1.0～10 μg/L;검출한위0.1 μg/L(S/N=3).차방법구유고적령민도화은정성,가응용우식품중황곡매독소적측정.
In the presence of hydrochloric acid, tetraethoxysilicane was hydrolyzed and formed silica sol. Non-labeled immunosensor was fabricated by droping the mixture solution of the silica sol and antibody of aflatoxin B_1 on the surface of glassy carbon electrode. In this work, a Fe(CN)_6~(3-/4-) phosphate buffer solution) was employed as base solution for investigating cyclic voltammetry(CV) and electrochemical impedance spectroscopic(EIS) performances of the sensor, respectively. The experimental results t indicated that because of the complex formed by the immunoreaction hindered the diffusion of Fe(CN)_6~(3-/4-) on the electrode surface, the redox peak current of the immunosensor in CV obviously decreased, and its electron transfer impedance linearly) increased with increasing the concentration of aflantoxin B_1(AFB). When the medium acidit and incubation) time were pH 6.5 and 20 min, respectively, the biggest electron transfer impedance changed value before and after the immunoreaction was obtained. Under the optimal conditions, a linear range to concentration of aflatoxin B_1 was 1-10 μg/L with a detection limit of 0.1 μg/L(S/N=3). Proposed method is of high sensitivity and stability, it has been successfully applied to determine AFB_1 in maize, rice and peanut.