CAJ | 학술논문

建立了检测氯胺酮的生物素-亲和素放大酶联免疫吸附测定法(BA-ELISA).实验最佳测定条件:抗原包被浓度为2.0 mg/L、氯胺酮单克隆抗体浓度为10.2 mg/L,生物素化羊抗小鼠IgG(Biotin-Igc)和酶标链霉亲和素(SA-HRP)的最佳反应浓度分别为0.29和1.0 mg/L.在此优化条件下,方法的线性范围为0.1-1000μg/L;检出限为0.03 μg/L.氯胺酮生物样品的加标回收率为94%～102%.与酶标二抗体系ELISA法相比,BA-ELISA具有更高的灵敏度,适于低浓度氯胺酮的检测.
건립료검측록알동적생물소-친화소방대매련면역흡부측정법(BA-ELISA).실험최가측정조건:항원포피농도위2.0 mg/L、록알동단극륭항체농도위10.2 mg/L,생물소화양항소서IgG(Biotin-Igc)화매표련매친화소(SA-HRP)적최가반응농도분별위0.29화1.0 mg/L.재차우화조건하,방법적선성범위위0.1-1000μg/L;검출한위0.03 μg/L.록알동생물양품적가표회수솔위94%～102%.여매표이항체계ELISA법상비,BA-ELISA구유경고적령민도,괄우저농도록알동적검측.
A rapid and sensitive method based on biotin-avidin mediated competitive enzyme-linked immu nosorbent assay(BA-ELISA) was established for the determination of ketamine.The optimal concentration of coated antigen and anti-ketamine monoclonal antibody were found to be 2.0 and 10.2 mg/L.The concentra tions of biotinylated goat anti-mouse IgG(Biotin-IgG) and streptavidin-horseradish peroxidase(SA-HRP) were optimized and the optimum results were found to be 0.29 and 1.0 mg/L, respectively.The linear range of the presented method was from 0.1 to 1000 μg/L, and the limit of detection was found to be 0.03 μg/L.The recoveries of ketamine spiked in human serum and urine were between 94% and 102%.Comparing the result of traditional ELISA, the present BA-ELISA method had a lower detection limit for ketamine.The experimental results indicated that the present BA-ELISA method was specific and sensitive.