CAJ | 학술논문

采用沉淀聚合法“原位”(in-situ)聚合合成了交联聚甲基丙烯酸缩水甘油酯连续床色谱柱，对其进行化学改性后，分别得到含有11个碳原子间隔臂以及不含间隔臂键合了蛋白A的高效亲合色谱柱。考察了这两种色谱介质的性能，结果表明含有间隔臂的介质有一定的疏水性；对传统制备蛋白配基的亲合色谱介质的合成路线进行了改进；采用不含间隔臂的亲合柱测定了人血浆中人免疫球蛋白G(HIgG)的含量，所测得的定量标准曲线线性相关系数达到0.999；考察了流速对所合成的连续床色谱柱柱压的影响，当流速高达9.0mL/min时，柱压也仅为6.5MPa；采用pH梯度对HIgG的不同亚基进行了分离。
채용침정취합법“원위”(in-situ)취합합성료교련취갑기병희산축수감유지련속상색보주，대기진행화학개성후，분별득도함유11개탄원자간격비이급불함간격비건합료단백A적고효친합색보주。고찰료저량충색보개질적성능，결과표명함유간격비적개질유일정적소수성；대전통제비단백배기적친합색보개질적합성로선진행료개진；채용불함간격비적친합주측정료인혈장중인면역구단백G(HIgG)적함량，소측득적정량표준곡선선성상관계수체도0.999；고찰료류속대소합성적련속상색보주주압적영향，당류속고체9.0mL/min시，주압야부위6.5MPa；채용pH제도대HIgG적불동아기진행료분리。
Reactive continuous rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) have been prepared by “in-situ” copolymerization of the monomers in the presence of porogenic dilute,and protein A was immobilized on the continuous rods directly or through a 11 carbon atom space arm with molded synthetic methods.The properties of these two rods columns were characterized and the results showed that the medium with space arm had some extent hydrophobicity.The affinity column without space arm was used to determine the human immunoglobulin G (HIgG) in human serum.The correlative coefficient of the calibration curve was 0.999.The effect of the flow rate on the pressure of the continuous rods was also investigated.The subclasses of HIgG were separated by the use of a pH gradient.