CAJ | 학술논문

分别将β-环糊精、2，6-二甲基-β-环糊精和2，3，6-三甲基-β-环糊精作为手性流动相添加剂，系统地研究了D，L-乳酸在反相HPLC系统中的拆分，考察了流动相种类，pH值和手性流动相添加剂的浓度对手性分离的影响，建立了甲基化β-环糊精动态手性固定相法分离乳酸对映体的方法。
분별장β-배호정、2，6-이갑기-β-배호정화2，3，6-삼갑기-β-배호정작위수성류동상첨가제，계통지연구료D，L-유산재반상HPLC계통중적탁분，고찰료류동상충류，pH치화수성류동상첨가제적농도대수성분리적영향，건립료갑기화β-배호정동태수성고정상법분리유산대영체적방법。
The enantiomeric resolution of lactic acid was studied by reversed high performance liquid chromatography. β-cyclodextrin (β-CD), dimethyl (DM) and TM-β-CD were used as chiral mobile phase additives. The effects of different mobile phase, pH and concentration of chiral mobile phase additives on resolution of D,L-lactic acid were investigated. The results showed: the enantioselectivity of TM-β-CD was better than those of β-CD and DM-β-CD. D,L-lactic acid could be partly separated by DM-β-CD 　and could not be separated by β-CD, while it could be fully separated by TM-β-CD. As the concentration of TM-β-CD changed from 0.20　mmol/L to 1.00　mmol/L, The resolution varied slightly. The experiment of the effect of pH of mobile phase on Rs showed: as pH changed from 2.4 to 3.0, the Rs remain stable, and when pH＞3.1, the Rs decreased with the increase of pH. The RSD of retention time of L-lactic acid and D-lactic acid were 0.2% and 0.2%, respectively. The chromatographic systems with a dynamically-generated stationary phase with 2,3,6-tri-O-methyl-β-cyclodextrin proved to be an effective method for lactic acid enantiomer separation