CAJ | 학술논문

研制了含有nm级Ag颗粒的葡萄糖氧化酶电极.结果表明,该电极具有很大的响应电流,在相同的条件下,其响应电流比文献所报导的含有Au或SiO2的酶电极响应电流大.当颗粒粒径为4.3～7.8nm时,酶电极响应电流随粒径的增大而增大;而当粒径继续增大时,其响应电流则随粒径的增大而减小.对Ag颗粒在酶电极中的作用机理也进行了初步探讨.
연제료함유nm급Ag과립적포도당양화매전겁.결과표명,해전겁구유흔대적향응전류,재상동적조건하,기향응전류비문헌소보도적함유Au혹SiO2적매전겁향응전류대.당과립립경위4.3～7.8nm시,매전겁향응전류수립경적증대이증대;이당립경계속증대시,기향응전류칙수립경적증대이감소.대Ag과립재매전겁중적작용궤리야진행료초보탐토.
An amperometric enzyme electrode based on immobilization of glucose oxidase(GOD) within silver(Ag) nanoparticles is presented. The enzyme electrodes are platinum electrodes covered with a membrane containing GOD and Ag nanoparticles. The results show that this kind of enzyme electrodes possess higher sensitivity than those containing Au or SiO2 nanoparticles. Comparing the response current of these Ag-GOD electrodes, it is found that the sensitivity of the enzyme electrodes increases with increasing Ag size in the case of 4.3-7.8nm. In the case of Ag size increasing continously, the situation is different, the enzyme electrodes have lower sensitivity with increasing Ag size. The mechanism of this phenomenon is studied and discussed on the basis of the ability of Ag nanoparticles transfer electron and the adsorption of GOD onto ultrafine particles.