CAJ | 학술논문

在λex/λem=450/556nm，十二烷基苯磺酸钠(DBS)存在下，吖啶橙-罗丹明6G能够发生有效能量转移，使罗丹明6G荧光大大增强；酸性条件下，正磷酸根与钼酸盐反应生成磷钼酸，磷钼酸与R6G形成离子缔合物，使R6G的荧光猝灭。利用吖啶橙-罗丹明6G能量转移荧光法测定痕量磷，提高了测定磷的灵敏度和选择性。磷含量在0.05～0．70 μg/L范围内与罗丹明6G的荧光猝灭程度呈良好的线性关系。最低检出限为5ng/L；回收率为95％～110％；测定0.50μg/L磷溶液；相对标准偏差为6%。该法用于水样及土壤中活性磷的测定，结果满意。
재λex/λem=450/556nm，십이완기분광산납(DBS)존재하，아정등-라단명6G능구발생유효능량전이，사라단명6G형광대대증강；산성조건하，정린산근여목산염반응생성린목산，린목산여R6G형성리자체합물，사R6G적형광졸멸。이용아정등-라단명6G능량전이형광법측정흔량린，제고료측정린적령민도화선택성。린함량재0.05～0．70 μg/L범위내여라단명6G적형광졸멸정도정량호적선성관계。최저검출한위5ng/L；회수솔위95％～110％；측정0.50μg/L린용액；상대표준편차위6%。해법용우수양급토양중활성린적측정，결과만의。
It was found that at the condition of λex/ λem=450／556 nm，in thedodecyl benzene sodium sulfonate solution, the effective energy transfer could occur between AO and R6G. The fluorescence intensity of R6G was diminished by molybdophosphate which was formed by the reaction between orthophosphate and molybdate in hydrochloric acid medium. The detection limit of this method was 5 ng/L. The range of determination for phosphorous was 0.05～0．70 μg/L. The relative standard deviation for the determination of 0.5 μg/L phosphorous was 6% (n=13). The recoveries for the addition of 0.2～0．4 μg/L phosphorous were 95%～110％. The method has been applied to the determination of phosphorous in water samples and soil samples with satisfactory reuslts.