中外健康文摘
中外健康文摘
중외건강문적
WORLD HEALTH DIGEST
2009年
18期
82-84
,共3页
脓毒症%血小板膜糖蛋白CD62p%CD63%血清前降钙素%C反应蛋白
膿毒癥%血小闆膜糖蛋白CD62p%CD63%血清前降鈣素%C反應蛋白
농독증%혈소판막당단백CD62p%CD63%혈청전강개소%C반응단백
sepsis Platelet membrane glycoproteins CD62p CD63 PCT CRP
目的 观察重症脓毒症患者血小板膜糖蛋白CD62p、CD63表达水平的变化,探讨两者与重症脓毒症严重程度及预后的关系.方法 用荧光素标志的单克隆抗体和流式细胞仪检测40例重症脓毒症患者发病后不同时间血小板糖蛋白CD62p、CD63的分子表达,采用酶联免疫吸附法(ELISA)测定发病后24 h内血清前降钙素(PCT)和C反应蛋白(CRP)浓度, 并且进行相关性分析.结果 重症脓毒症患者发病后各时间点CD62p、CD63水平较正常对照均显著升高( P均<0.01);死亡组患者各时间点CD62p、CD63水平较存活组均升高(P 均<0.05).CD62p、CD63水平与PCT、CRP呈正相关.结论 重症脓毒症患者血小板处于高激活状态, CD62p、CD63水平均显著 升高,可以作为判断疾病严重程度及预后的指标.
目的 觀察重癥膿毒癥患者血小闆膜糖蛋白CD62p、CD63錶達水平的變化,探討兩者與重癥膿毒癥嚴重程度及預後的關繫.方法 用熒光素標誌的單剋隆抗體和流式細胞儀檢測40例重癥膿毒癥患者髮病後不同時間血小闆糖蛋白CD62p、CD63的分子錶達,採用酶聯免疫吸附法(ELISA)測定髮病後24 h內血清前降鈣素(PCT)和C反應蛋白(CRP)濃度, 併且進行相關性分析.結果 重癥膿毒癥患者髮病後各時間點CD62p、CD63水平較正常對照均顯著升高( P均<0.01);死亡組患者各時間點CD62p、CD63水平較存活組均升高(P 均<0.05).CD62p、CD63水平與PCT、CRP呈正相關.結論 重癥膿毒癥患者血小闆處于高激活狀態, CD62p、CD63水平均顯著 升高,可以作為判斷疾病嚴重程度及預後的指標.
목적 관찰중증농독증환자혈소판막당단백CD62p、CD63표체수평적변화,탐토량자여중증농독증엄중정도급예후적관계.방법 용형광소표지적단극륭항체화류식세포의검측40례중증농독증환자발병후불동시간혈소판당단백CD62p、CD63적분자표체,채용매련면역흡부법(ELISA)측정발병후24 h내혈청전강개소(PCT)화C반응단백(CRP)농도, 병차진행상관성분석.결과 중증농독증환자발병후각시간점CD62p、CD63수평교정상대조균현저승고( P균<0.01);사망조환자각시간점CD62p、CD63수평교존활조균승고(P 균<0.05).CD62p、CD63수평여PCT、CRP정정상관.결론 중증농독증환자혈소판처우고격활상태, CD62p、CD63수평균현저 승고,가이작위판단질병엄중정도급예후적지표.
Objective To investigate the changes of the platelet glycoprotein CD62p、CD63 in severe sepsis patients,and their relationship with severity and prognosis of patients. Methods CD62p、CD63 expressions were measured by flow cytometry(FCM)and platelet monoclonal antibodies at dif fer ent periods after the onset of severe sepsis in 40 patients,Serum concentrations of procalcitonin(PCT) and C-Reactive Protein (CRP) were determined within 24 hours of the onset of severe sepsis. The relationship between all the de ter mi na tions and the clinical picture and prognosis of the patients was analyzed. Result The expressions of the platelet gly co pro tein CD62p、CD63 levels in severe sepsis group was significantly higher than normal group(P<0.01). CD62p、CD63 levels were higher in non-survivors (P<0.05). There was significant positive correlation between the platelet gly co pro tein CD62p、CD63 levels and PCT and CRP. Con clu sion Platelets were significantly activated in septic patients,and the degree of platelet activation was correlated with the severity of sepsis and used a prognostic marker.
