CAJ | 학술논문

结合PCR扩增和cDNA文库筛选方法，本文从人胎盘cDNA文库中分离到一个与细胞周期调节有关的NEK6基因（GenBank登录号：AF087909）。该基因的cDNA序列全长1590 bp，编码一种由312氨基酸组成的蛋白质。在催化功能区，NEK6与小鼠的NEK1具有39.32%的同源性。Northern 印迹证实在人体大多数组织中，NEK6基因均能表达1.6kb、3.0kb和9.5kb 3种mRNA转录物，其中1.6kb的表达产物可能是NEK6基因的实际表达产物，并且其在卵巢中的表达量明显高于睾丸组织。通过Radiation hybrid分析，NEK6基因进一步被定位于人9号染色体。结果初步显示，NEK6基因是哺乳动物NEK基因家族中的重要新成员，并可能在细胞分裂中发挥调节作用。
결합PCR확증화cDNA문고사선방법，본문종인태반cDNA문고중분리도일개여세포주기조절유관적NEK6기인（GenBank등록호：AF087909）。해기인적cDNA서렬전장1590 bp，편마일충유312안기산조성적단백질。재최화공능구，NEK6여소서적NEK1구유39.32%적동원성。Northern 인적증실재인체대다수조직중，NEK6기인균능표체1.6kb、3.0kb화9.5kb 3충mRNA전록물，기중1.6kb적표체산물가능시NEK6기인적실제표체산물，병차기재란소중적표체량명현고우고환조직。통과Radiation hybrid분석，NEK6기인진일보피정위우인9호염색체。결과초보현시，NEK6기인시포유동물NEK기인가족중적중요신성원，병가능재세포분렬중발휘조절작용。
Integrated with PCR and screening cDNA library, a novel gene, NEK6 related to cell cycle regulation (GeneBank Accession Number: AF087909), was isolated from human placenta cDNA library. The cDNA sequence of NEK6 is 1590bp, encoding a 312 amino acid protein. In the catalytic domains, NEK6 was identical to the rat NEK1, sharing with 39.32% identity. Northern blot showed that NEK6 gene expressed three transcripts in most human tissues and the actual NEK6 gene expressed transcript was 1.6 kb in length. NEK6 gene was mapped to human chromosome 9 by radiation hybrid. Preliminary results indicate that NEK6 gene belongs to the NEK gene family and may function in the cell divisions.