山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2001年
6期
10-11
,共2页
朱传升%毕可红%刘秀兰
硃傳升%畢可紅%劉秀蘭
주전승%필가홍%류수란
白血病%基因%P15%失活%甲基化
白血病%基因%P15%失活%甲基化
백혈병%기인%P15%실활%갑기화
用聚合酶链反应（PCR）方法扩增P15基因外显子1，再用限制性内切酶-PCR方法检测P15基因甲基化。结果48例患者中P15基因失活者共27例（58.92％），ALL21例中有12例（57.41％），ANLL27例中15例（55.56％）。P15基因以甲基化失活为主。P15CpG岛甲基化在各型急性白血病中均有很高的发生率，可以作为各型急性白血病通用的微量残留病（MRD）指标；白血病缓解期P15CpG岛甲基化仍为阳性可能预示着复发。认为P15基因失活的检测对于探讨急性白血病的发病机制、判断预后有重要意义。
用聚閤酶鏈反應（PCR）方法擴增P15基因外顯子1，再用限製性內切酶-PCR方法檢測P15基因甲基化。結果48例患者中P15基因失活者共27例（58.92％），ALL21例中有12例（57.41％），ANLL27例中15例（55.56％）。P15基因以甲基化失活為主。P15CpG島甲基化在各型急性白血病中均有很高的髮生率，可以作為各型急性白血病通用的微量殘留病（MRD）指標；白血病緩解期P15CpG島甲基化仍為暘性可能預示著複髮。認為P15基因失活的檢測對于探討急性白血病的髮病機製、判斷預後有重要意義。
용취합매련반응（PCR）방법확증P15기인외현자1，재용한제성내절매-PCR방법검측P15기인갑기화。결과48례환자중P15기인실활자공27례（58.92％），ALL21례중유12례（57.41％），ANLL27례중15례（55.56％）。P15기인이갑기화실활위주。P15CpG도갑기화재각형급성백혈병중균유흔고적발생솔，가이작위각형급성백혈병통용적미량잔류병（MRD）지표；백혈병완해기P15CpG도갑기화잉위양성가능예시착복발。인위P15기인실활적검측대우탐토급성백혈병적발병궤제、판단예후유중요의의。
To investigate the role of P15 gene in the pathegenesis ofleukemia，48 patients with newly diagnosed acute leukemia were studied.PCR technique was used to detect homozygous of P15 gene，restriction enzyme-PCR technique used to detect gene methylation.This results showed P15 gene inactivation was detected in 28 of the 48 patients.For all patients，methylation was the major pathway of P15 gene inactivation.Patients with P15 gene inactivation had a poor response to chemotherapy and a lower remission.This suggests that the methylation of P15 gene islands occurs very commonly in every types of AL.It can be used as a gene marker for AL and for minimal residual disease in CR.The inactivation of P15 gene plays a key role in the pathogenesis of acute leukemia.
