CAJ | 학술논문

摘要运用高效液相色谱手性流动相法(HPLC-CMP)对影响甲状腺素对映体(D-,L-T4)分离方法的因素：三乙胺(TEA)浓度，流动相pH值，铜离子(Cu2＋）浓度，L-脯氨酸(L-pro)浓度，柱温以及流动相的流速进行了系统的研究。同时，考察了色谱方法分离T4对映体的线性关系，精密度和准确度。线性响应范围为0.6～3.2 nmol (D-，L-T4），线性相关系数为rD-T4=0.9980，rL-T4=0.9990，日内和日间的精密度分别为RSD＜2.3%(n=6)，RSD＜3.15％（n=5)。结果表明本实验所得的色谱条件较文献报道的优越，分离条件简单，重现性好。HPLC-CMP法测定甲状腺素对映体其意义在于该方法可为定量测定药品及人体血液中D-，L-T4两种异构体，为治疗药物监测(TDM)和药物不良反应监测(ADRs)提供了依据。
적요운용고효액상색보수성류동상법(HPLC-CMP)대영향갑상선소대영체(D-,L-T4)분리방법적인소：삼을알(TEA)농도，류동상pH치，동리자(Cu2＋）농도，L-포안산(L-pro)농도，주온이급류동상적류속진행료계통적연구。동시，고찰료색보방법분리T4대영체적선성관계，정밀도화준학도。선성향응범위위0.6～3.2 nmol (D-，L-T4），선성상관계수위rD-T4=0.9980，rL-T4=0.9990，일내화일간적정밀도분별위RSD＜2.3%(n=6)，RSD＜3.15％（n=5)。결과표명본실험소득적색보조건교문헌보도적우월，분리조건간단，중현성호。HPLC-CMP법측정갑상선소대영체기의의재우해방법가위정량측정약품급인체혈액중D-，L-T4량충이구체，위치료약물감측(TDM)화약물불량반응감측(ADRs)제공료의거。
A high performance liquid chromatographic (HPLC) method using triethylamine (TEA), copper *" acetate and L-proline as the chiral mobile phase (CMP) and the enantiomric analysis of thyroxine (T4) are described. The influence of the mobile phase parameters including the TEA concentration, manipulation pH, concentration of Cu*", concentration of L-proline, temperature of column and flow rate of eluent on the enantiomers separation of thyroxine was studied. The HPLC-CMP is a simple method for separation and determination of D，L-T4 in drug or in human plasma. It can be used to therapeutic drug or adverse drug reactions monitor.