CAJ | 학술논문

硫酸介质中，高锰酸钾可以氧化甲氧苄啶发生化学发光反应，硫代硫酸钠对这一化学发光反应有极强的增敏作用。采用流动注射技术，建立了高锰酸钾-甲氧苄啶-硫代硫酸钠化学发光体系测定甲氧苄啶的化学发光新方法．考察了各种影响因素，确立了反应的最佳条件。方法的检出限为3.8×10－8 g/mL甲氧苄啶；线性响应范围为1×10－7～1×10－5 g/mL。对4×10－6 g/mL甲氧苄啶进行11次平行测定，相对标准偏差为1.8%。方法已成功地用于片剂中甲氧苄啶含量的测定，结果与药典标准法测得值一致。
류산개질중，고맹산갑가이양화갑양변정발생화학발광반응，류대류산납대저일화학발광반응유겁강적증민작용。채용류동주사기술，건립료고맹산갑-갑양변정-류대류산납화학발광체계측정갑양변정적화학발광신방법．고찰료각충영향인소，학립료반응적최가조건。방법적검출한위3.8×10－8 g/mL갑양변정；선성향응범위위1×10－7～1×10－5 g/mL。대4×10－6 g/mL갑양변정진행11차평행측정，상대표준편차위1.8%。방법이성공지용우편제중갑양변정함량적측정，결과여약전표준법측득치일치。
A chemiluminescence method based on the chemiluminescence reaction of KMnO4-trimethoprin (TMP)-Na2S2O3 for the determination of TMP with flow injection technique was developed. The main facts which affected the chemiluminescence reaction were examined. Under optimum conditions, the linear range for the determination of TMP was 1×10－7～1×10－5 g/mL and the detection limit was 3．8×10－8 g/mL. The relative standard deviation for the determination of 4×10－6 g/mL TMP (n=11) was 1.8%. The method has been used to determine the TMP in tablets and the results were in good agreement with those obtained by classical method