CAJ | 학술논문

在反相液相色谱（RPLC）中用Snyder经验方程和计量置换保留模型（SDM-R）中的参数对溶质为脂肪醇同系物，流动相为脂肪酸同系物时溶质的保留行为进行了研究，结果表明用SDM-R参数具有明显的优越性。另外，由于用Snyder经验公式中二参数之间的作图无法准确求得斜率，且其不具有明确的物理意义，而由SDM-R二参数作图，不但能准确求得斜率j（与1 mol溶剂和固定相结合能有关的常数），而且j具有明确的物理意义并符合碳数规律，所以，参数j有可能用于RPLC中表征溶剂强度。由此得出，在RPLC中，对同一置换剂而言，随同系物溶质的Z(1 mol溶剂化溶质被溶剂化固定相吸附时，从二者接触面释放出的置换剂分子数)和logI(与1 mol溶质和固定相亲和势有关的常数)值增大，它们的保留时间也增大；对同一溶质而言，随着在同系物置换剂中碳链的增长，Z和logI值的减小，它的保留时间也缩短。同时还可得出，随着同系物置换剂j值的减小，它们的洗脱能力也增强。
재반상액상색보（RPLC）중용Snyder경험방정화계량치환보류모형（SDM-R）중적삼수대용질위지방순동계물，류동상위지방산동계물시용질적보류행위진행료연구，결과표명용SDM-R삼수구유명현적우월성。령외，유우용Snyder경험공식중이삼수지간적작도무법준학구득사솔，차기불구유명학적물리의의，이유SDM-R이삼수작도，불단능준학구득사솔j（여1 mol용제화고정상결합능유관적상수），이차j구유명학적물리의의병부합탄수규률，소이，삼수j유가능용우RPLC중표정용제강도。유차득출，재RPLC중，대동일치환제이언，수동계물용질적Z(1 mol용제화용질피용제화고정상흡부시，종이자접촉면석방출적치환제분자수)화logI(여1 mol용질화고정상친화세유관적상수)치증대，타문적보류시간야증대；대동일용질이언，수착재동계물치환제중탄련적증장，Z화logI치적감소，타적보류시간야축단。동시환가득출，수착동계물치환제j치적감소，타문적세탈능력야증강。
The retention behaviours of n-aliphatic alcohols with mobilephase of n-aliphatic acids-water in reversed-phase liquid chromatography (RPLC) were studied with stoichiometric displacement model for retention (SDM-R) and Snyder′s empirical equation. The results obtained showed that SDM-R was superior to Snyder′s empirical equation when they were employed to characterize the retention behaviours of homologue and the solvent strength of homologous displacers. The slope, 1/q, of the plot of logK0w versus S has no clear physical meaning and can not be measured accurately, while the slope, j (a constant relating to the affinity of 1 mole solvent to bonded stationary phase ), of the plot of logI (a constant relating to the affinity of 1 mole of solute to bonded stationary phase) versus Z (the number of solvent molecules released from the contact surface region of 1 mole of solvated solute absorbed onto solvated bonded stationary phase) is not only determined accurately but also has a clear physical meaning and follows the rule of carbon number. Therefore, if it is possible, j can be used to characterize the solvent strength employed in RPLC. The conclusion is that with a given displacer, the retention of homologue in RPLC increases with their Z and logI increasing. If a solute is given, with the increment in carbon chain of homologous displacers, the values of Z and logI of the solute decrease and thus lead to the decrease of the solute retention, and the elution strength of a solvent becomes strong as the j value of a homologous displacer becomes small.