分析化学
分析化學
분석화학
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2001年
2期
138-141
,共4页
毛细管电泳微系统%安培检测%多巴胺
毛細管電泳微繫統%安培檢測%多巴胺
모세관전영미계통%안배검측%다파알
将电极、6cm分离毛细管、缓冲池、检测池集成于8.4×5.0cm有机玻璃片上，制作了一个毛细管电泳微系统。以碳纤维微盘电极作为工作电极，采用三电极体系柱端检测了1×10-4mol/L多巴胺(DA)，具有良好的重现性，检测限3.6×10-8 mol/L,线性范围5×10-7～1×10-4mol/L,并在该系统上分离了邻苯二酚(CA)和多巴胺的混合物。
將電極、6cm分離毛細管、緩遲池、檢測池集成于8.4×5.0cm有機玻璃片上，製作瞭一箇毛細管電泳微繫統。以碳纖維微盤電極作為工作電極，採用三電極體繫柱耑檢測瞭1×10-4mol/L多巴胺(DA)，具有良好的重現性，檢測限3.6×10-8 mol/L,線性範圍5×10-7～1×10-4mol/L,併在該繫統上分離瞭鄰苯二酚(CA)和多巴胺的混閤物。
장전겁、6cm분리모세관、완충지、검측지집성우8.4×5.0cm유궤파리편상，제작료일개모세관전영미계통。이탄섬유미반전겁작위공작전겁，채용삼전겁체계주단검측료1×10-4mol/L다파알(DA)，구유량호적중현성，검측한3.6×10-8 mol/L,선성범위5×10-7～1×10-4mol/L,병재해계통상분리료린분이분(CA)화다파알적혼합물。
We have made a cheap microsystem of capillary electrophoresis with a new method, integrating the electrodes, injection channel, separation channel, buffer reservoirs and detection cell on a polymethylmethacrylate (PMMA) chip. Using an integrated micro carbon fiber disk electrode as the working electrode in three electrodes system, 1 × 10-4 mol/L dopamine (DA) could be detected with end-column amperometric detection. The reproducibility was good. Peak current was 6.73 nA、theoretical plate numher was 71300/m and height equivalent of one theoretical plate height was 14.0 μm for 1 × 10-4 mol/L DA. The limit of detection was 3.6× 10-8mol/L and the linear range was extended from 5 × 10-7 mol/L to 1 × 10-4 mol/L for DA. 1 × 10-4 mol/L catechol (CA) and 5× 10- 5 mol/L DA were also separated completely with R. = 10.1.
