CAJ | 학술논문

目的:建立净宫颗粒中总皂苷的含量测定方法.方法:色谱柱为C18柱,以乙腈为流动相A,水为流动相B,进行梯度洗脱;检测波长为203 nm.结果:三七皂苷R1在0.16～6.18 μg,人参皂苷Rg1在0.41～16.42 μg,人参皂苷Rb1在0.37～14.86 μg之间,进样量与峰面积线性关系良好.三七皂苷R1平均回收率99.3%,人参皂苷Rg1平均回收率100.0%,人参皂苷Rb1平均回收率99.4%.结论:本法可准确测定净宫颗粒中总皂苷的含量.
목적:건립정궁과립중총조감적함량측정방법.방법:색보주위C18주,이을정위류동상A,수위류동상B,진행제도세탈;검측파장위203 nm.결과:삼칠조감R1재0.16～6.18 μg,인삼조감Rg1재0.41～16.42 μg,인삼조감Rb1재0.37～14.86 μg지간,진양량여봉면적선성관계량호.삼칠조감R1평균회수솔99.3%,인삼조감Rg1평균회수솔100.0%,인삼조감Rb1평균회수솔99.4%.결론:본법가준학측정정궁과립중총조감적함량.
Objective:To establish a method for the determination of total saponins in Jinggong Granules.Methods:The C18 column was used.The mobile phase consisted of acetonitrile and water using gradient elution.The UV detector was set at 203nm.Results:The cali-bration curves of notoginsenoside R1 showed good linearity over the range of 0.16～6.18 μg,the average recovery rote was 99.3%.The cali-bration curves of notoginsenoside Rg1 showed good linearity over the range of 0.41～16.42 μg,the average recovery rote was 100%.The calibration curves of notoginsenoside Rb, showed good linearity over the range of 0.37～14.86 μg,the average recovery rate was 99.4%. Conclusion:The method can be used to determine the total saponins in Jinggong Granules.