遗传
遺傳
유전
HEREDITAS(BEIJING)
2009年
11期
1149-1157
,共9页
生物信息学%玉米%microRNA%预测%靶基因
生物信息學%玉米%microRNA%預測%靶基因
생물신식학%옥미%microRNA%예측%파기인
bioinformatics%maize%microRNA%prediction%target gene
microRNAs(miRNAs)是一类非编码的小分子RNA,通过碱基互补调控靶基因的表达.鉴定和发现新的miRNAs及其靶基因,对揭示miRNAs在基因表达调控中的作用至关重要.玉米全基因组测序工作开展较晚,已经鉴定登记的miRNAs很少,对靶基因的调控作用尚待解明.文章根据miRNA进化上的保守性,以已知的植物miRNAs为探针,与相关数据库中玉米表达序列标签(EST)和基因组序列(GSS)中的非编码序列比对,共发现11个新的miRNA前体.虽然在序列长度和二级结构方面各有变化,但这11个前体均可折叠形成miRNA家族的标准二级结构.通过靶基因预测,找到其中7条miRNAs的26个靶基因,分别编码与新陈代谢、信号转导、转录调节、跨膜运输、生物和非生物胁迫及叶绿体组装等相关的蛋白.这些miRNAs及其靶基因的鉴定,补充了miRNA数据库的不足.
microRNAs(miRNAs)是一類非編碼的小分子RNA,通過堿基互補調控靶基因的錶達.鑒定和髮現新的miRNAs及其靶基因,對揭示miRNAs在基因錶達調控中的作用至關重要.玉米全基因組測序工作開展較晚,已經鑒定登記的miRNAs很少,對靶基因的調控作用尚待解明.文章根據miRNA進化上的保守性,以已知的植物miRNAs為探針,與相關數據庫中玉米錶達序列標籤(EST)和基因組序列(GSS)中的非編碼序列比對,共髮現11箇新的miRNA前體.雖然在序列長度和二級結構方麵各有變化,但這11箇前體均可摺疊形成miRNA傢族的標準二級結構.通過靶基因預測,找到其中7條miRNAs的26箇靶基因,分彆編碼與新陳代謝、信號轉導、轉錄調節、跨膜運輸、生物和非生物脅迫及葉綠體組裝等相關的蛋白.這些miRNAs及其靶基因的鑒定,補充瞭miRNA數據庫的不足.
microRNAs(miRNAs)시일류비편마적소분자RNA,통과감기호보조공파기인적표체.감정화발현신적miRNAs급기파기인,대게시miRNAs재기인표체조공중적작용지관중요.옥미전기인조측서공작개전교만,이경감정등기적miRNAs흔소,대파기인적조공작용상대해명.문장근거miRNA진화상적보수성,이이지적식물miRNAs위탐침,여상관수거고중옥미표체서렬표첨(EST)화기인조서렬(GSS)중적비편마서렬비대,공발현11개신적miRNA전체.수연재서렬장도화이급결구방면각유변화,단저11개전체균가절첩형성miRNA가족적표준이급결구.통과파기인예측,조도기중7조miRNAs적26개파기인,분별편마여신진대사、신호전도、전록조절、과막운수、생물화비생물협박급협록체조장등상관적단백.저사miRNAs급기파기인적감정,보충료miRNA수거고적불족.
MicroRNAs (miRNAs) are an extensive class of tiny RNA molecules that regulate the expression of target genes by means of complementary base pair interactions. Identification of miRNAs and their target genes is essential to understand the regulation network of miRNAs in gene expression. With the method of bioinformatic computation, we used previously deposited miRNA sequences from Arabidopsis, rice, and other plant species to blast the databases of maize expressed sequence tags and genomic survey sequence that do not correspond to protein coding genes. A total of 11 novel miRNAs were identified from maize following a range of filtering criteria. All the potential miRNA precursors can be folded into the typical secondary structure of miRNA family, despite of variation in length and structure. Using these miRNAs sequences, we further blasted the databases of maize mRNAs and identified 26 target genes for seven of the eleven newly identified miRNAs. These genes encode twenty-six proteins involved in metabolism, signal transduction, transcrip-tional regulation, transmembrane transport, biostress, and abiostress responses, as well as chloroplast assembly. The identification of these novel miRNAs is a useful complement to the maize miRNA database
