CAJ | 학술논문

建立了一种新的微乳体系,用于微乳液相色谱同时分析山楂叶提取物中牡荆素鼠李糖苷、芦丁、牡荆素及金丝桃苷4种黄酮成分.通过对影响分离选择性的主要因素进行考察,得到最佳微乳体系的组成为: 1.0%(w/w) 聚氧乙烯月桂醇(Brij35)-1.1%(w/w)正丁醇-0.1%(w/w)正辛醇-0.3%三乙胺(V/V)(H_3PO_4调节至pH 2.5).在此微乳体系中,表面活性剂类型和浓度、油相种类、添加剂三乙胺、流动相的pH值对4种黄酮成分的分离起到了重要的作用.选择Venusil ASB C_(18)色谱柱(150 mm×4.6 mm, 5μm),流速为0.8 mL/min,检测波长为360 nm,柱温为35 ℃.结果显示,4种黄酮成分在27 min内达到基线分离,在0.95～140.8 mg/L范围内,4个黄酮成分的线性相关系数r≥0.9995,平均回收率98.6%～101.6%.本方法可应用于山楂叶提取物中4种主要黄酮成分的质量分析.
건립료일충신적미유체계,용우미유액상색보동시분석산사협제취물중모형소서리당감、호정、모형소급금사도감4충황동성분.통과대영향분리선택성적주요인소진행고찰,득도최가미유체계적조성위: 1.0%(w/w) 취양을희월계순(Brij35)-1.1%(w/w)정정순-0.1%(w/w)정신순-0.3%삼을알(V/V)(H_3PO_4조절지pH 2.5).재차미유체계중,표면활성제류형화농도、유상충류、첨가제삼을알、류동상적pH치대4충황동성분적분리기도료중요적작용.선택Venusil ASB C_(18)색보주(150 mm×4.6 mm, 5μm),류속위0.8 mL/min,검측파장위360 nm,주온위35 ℃.결과현시,4충황동성분재27 min내체도기선분리,재0.95～140.8 mg/L범위내,4개황동성분적선성상관계수r≥0.9995,평균회수솔98.6%～101.6%.본방법가응용우산사협제취물중4충주요황동성분적질량분석.
A novel system was developed for the rapid determination of vitexin, vitexin-2″-O-rhamnoside, rutin, and hyperoside in the extract of hawthorn(Crataegus pinnatifida Bge.) leaves with microemulsion liquid chromatography(MELC). The effect on the chromatography of varying the operating paramers was studied. The optimized MELC system with microemulsion was consisting of 1.0%(w/w) brij35-1.1%(w/w) n-butanol-0.1%(w/w) n-octanol-0.3%(v/v) triethylamine, the pH was adjusted to 2.5 with phosphoric acid. The type and concentration of surfactant, types of oil phase, the pH and triethylamine as the organic additive in microemulsion played an important role for separation of four flavonoids. MELC analysis was performed on a Venusil ASB C_(18) analytical column (150 mm× 4.6 mm, i.d., 5 μm). The flow rate was set at 0.8 mL/min and the eluent was detected at 340 nm for the four flavonoids. The calibration curves of the four flavonoids are linear(r>0.9995) over the concentration range of 0.95-140.0 mg/L. The mean recoveries are 98.6% to 101.6%. The results indicate that the optimized method was successfully applied to the analysis of four important flavonoids in the extract of hawthorn leaves.