CAJ | 학술논문

目的探讨川芎嗪对急性百草枯(PQ)中毒大鼠所致肺损伤的治疗作用.方法采用腹腔注射20% PQ溶液制作大鼠急性PQ中毒模型,将大鼠随机分成空白组、阴性对照组、阳性对照组、川芎嗪低剂量组和川芎嗪高剂量组,观察大体标本、组织病理以及肺湿/干质量比、肺泡灌洗液(BALF)中性粒细胞比和蛋白含量.同时测定血清和BALF中MDA含量、SOD和GSH-Px活力以及肺组织核因子-кB(NF-кB)和iNOS活性.结果与阴性对照组相比,川芎嗪低剂量组肺组织淤血、水肿明显减轻,血浆和BALF中MDA含量明显下降(P均＜0.01);GSH-Px和SOD活力明显升高(P＜0.01).NF-кB和iNOS降低(P＜0.05,或＜0.01).结论川芎嗪可使急性PQ中毒引起的脂质过氧化损害得到改善,且能降低NF-кB及iNOS水平,减轻PQ中毒大鼠肺组织损伤.
목적 탐토천궁진대급성백초고(PQ)중독대서소치폐손상적치료작용.방법 채용복강주사20% PQ용액제작대서급성PQ중독모형,장대서수궤분성공백조、음성대조조、양성대조조、천궁진저제량조화천궁진고제량조,관찰대체표본、조직병리이급폐습/간질량비、폐포관세액(BALF)중성립세포비화단백함량.동시측정혈청화BALF중MDA함량、SOD화GSH-Px활력이급폐조직핵인자-кB(NF-кB)화iNOS활성.결과 여음성대조조상비,천궁진저제량조폐조직어혈、수종명현감경,혈장화BALF중MDA함량명현하강(P균＜0.01);GSH-Px화SOD활력명현승고(P＜0.01).NF-кB화iNOS강저(P＜0.05,혹＜0.01).결론 천궁진가사급성PQ중독인기적지질과양화손해득도개선,차능강저NF-кB급iNOS수평,감경PQ중독대서폐조직손상.
Objective To study the therapeutic effect of ligustrazine against acute lung injury by paraquat(PQ). Methods 50 SD rats were randomly divided into five experimental groups:blank group, negative control group, positive control group, ligustrazine low-dose group(LDG) and ligustrazine high-dose group(HDG). Macroscopic and histopathological examinations were performed and biological indexes were measured for the lung specimens. The indexes included lung wet weight/dry weight, the rats of neutrophils and protein content in the pulmonary alveolar lavage fluid. In the mean time, the levels of MDA, superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) both in plasma and iNOS in lung tissue homogenate were measured. Results Compared with negative control group, lung congestion and lung edema of ligustrazine low-dose group were mitigated. The level of MDA decreased in both plasma and BALF(P＜0.01), and and iNOS in lung tissue homogenate were significantly decreased (P＜0.05,＜0.01). Conclusions Administration of ligustrazine could improve the lipid peroxidation iNOS caused by acute poisoning of PQ.