CAJ | 학술논문

在碱性条件下，铁氰化钾氧化鲁米诺，产生化学发光，尿酸对该体系的化学发光有显著的增强作用(亚铁氰化钾存在时)。基于此，建立了一种直接测定尿酸的流动注射化学发光分析法。方法的线性范围为2.0×10－8～5.0×10－6 g/mL；检测限(3σ)为6.7×10－9 g/mL；相对标准偏差为1.1%(尿酸1.0×10－7 g/mL，n=11)。用于血清及尿样中尿酸的分析，结果令人满意。
재감성조건하，철청화갑양화로미낙，산생화학발광，뇨산대해체계적화학발광유현저적증강작용(아철청화갑존재시)。기우차，건립료일충직접측정뇨산적류동주사화학발광분석법。방법적선성범위위2.0×10－8～5.0×10－6 g/mL；검측한(3σ)위6.7×10－9 g/mL；상대표준편차위1.1%(뇨산1.0×10－7 g/mL，n=11)。용우혈청급뇨양중뇨산적분석，결과령인만의。
A flow injection chemiluminescence method for the determination of uric acid was described. The method is based on the chemiluminescence produced during the oxidation of luminol by hexacyanoferrate *# in an alkaline solution. The emission intensity is greatly enhanced by uric acid in the presence of hexacyanoferrate *" as suppressor. The flow system is very stable. the proposed method is simple, sensitive, and inexpensive. The chemiluminescence intensity is proportional to uric acid concentration over the range 2.0×10－8～5．0×10－6 g/mL with a detection limit of 6.7×10－9 g/mL. The relative standard deviation (RSD) in 1.1% (n=11). The method has been successfully applied to the determination of uric acid in human urine and serum samples. In addition, the possible reaction mechanism has been discussed as well.