CAJ | 학술논문

目的:提高利胆片的质量标准.方法:采用薄层色谱(TLC)法鉴别金银花、黄芩、知母及白芍,用高效液相色谱(HPLC)法测定方中大黄的含量.结果:TLC鉴别斑点明显.含量测定大黄素0.01064～0.3192 μg、大黄酚0.02586～0.7758 μg,进样量与峰面积线性关系良好.平均加样回收率大黄素为99.80%,RSD=0.78%(n=6);大黄酚为99.72%,RSD=1.18%(n=6).结论:建立的TLC鉴别和HPLC含量测定方法专属性强、重复性好,可用于利胆片的质量控制.
목적:제고리담편적질량표준.방법:채용박층색보(TLC)법감별금은화、황금、지모급백작,용고효액상색보(HPLC)법측정방중대황적함량.결과:TLC감별반점명현.함량측정대황소0.01064～0.3192 μg、대황분0.02586～0.7758 μg,진양량여봉면적선성관계량호.평균가양회수솔대황소위99.80%,RSD=0.78%(n=6);대황분위99.72%,RSD=1.18%(n=6).결론:건립적TLC감별화HPLC함량측정방법전속성강、중복성호,가용우리담편적질량공제.
Objective:To improve the quality standard of Lidan Tablets. Methods:TLC and HPLC were used.Results:The TLC method could indentify Flos Lonicerae,Scutellaria,Timosaponin and Paeony.The HPLC method could quantitatively determine the total content of emodin and chrysaphanol in Lidan Tablets.The linear range of emodin was 0.01064～0.3192 μg (r=0.9999) and the average re-covery was 99.8% with RSD of 0.78%;the linear range of chrysaphanol was 0.02586～0.7758 μg (r=0.9999) and the average recovery was 99.7% with RSD of 1.18%.Conclusion:The indentification methods are reproducible and specific.The quantitative determination method is simple and accurate.