CAJ | 학술논문

目的：观察玉屏风（ YPF）药液对环磷酰胺（ CY）抑制C57BL/6小鼠免疫功能的影响。方法腹部皮下注射环磷酰胺制作免疫抑制小鼠模型，分别使用玉屏风药液50 mg/d和100 mg/d给C57 BL/6小鼠灌胃，连续7 d。7 d以后，眼眶取血，血常规检测血细胞含量；制备脾淋巴细胞悬液，计数；流式细胞仪检测淋巴细胞亚群的含量；使用R848刺激细胞72 h， MTT法检测细胞的增殖情况。结果玉屏风药液可以抑制环磷酰胺诱导的小鼠血液中白细胞减少，部分缓解环磷酰胺所致的脾淋巴细胞减少，增加脾细胞中B细胞、NK细胞的含量，部分提高环磷酰胺免疫抑制小鼠脾淋巴细胞的增殖能力。结论玉屏风药液能够部分缓解环磷酰胺对C57 BL/6小鼠免疫功能的抑制作用。
목적：관찰옥병풍（ YPF）약액대배린선알（ CY）억제C57BL/6소서면역공능적영향。방법복부피하주사배린선알제작면역억제소서모형，분별사용옥병풍약액50 mg/d화100 mg/d급C57 BL/6소서관위，련속7 d。7 d이후，안광취혈，혈상규검측혈세포함량；제비비림파세포현액，계수；류식세포의검측림파세포아군적함량；사용R848자격세포72 h， MTT법검측세포적증식정황。결과옥병풍약액가이억제배린선알유도적소서혈액중백세포감소，부분완해배린선알소치적비림파세포감소，증가비세포중B세포、NK세포적함량，부분제고배린선알면역억제소서비림파세포적증식능력。결론옥병풍약액능구부분완해배린선알대C57 BL/6소서면역공능적억제작용。
Objective It is to observe the effect of Yupingfeng (YPF) solution on the immune function of C57BL/6 mice treated with Cyclophosphamide .Methods Cyclophosphamide was given to C57BL/6 mice by abdominal subcutaneous injection to establish inhibit immune function models which were given YPF solution at dose of 50mg/d and 100 mg/d respectively by intragastric administration for 7 days.Blood tests were used to count the percentage of blood cells in different groups of mice . Microscope was used to count spleen cells in different groups of mice .The cell surface stain method was used to observe the percentage of lymphocyte subsets .MTT assay was used to observe cell proliferation after simulation for 72 h with R848 .Re-sults YPF can inhibit Cyclophosphamide-induced neutropenia , partly inhibit the reduction of mouse spleen lymphocytes , in-crease the contents of B cell , NK cell, partly promote lymphocytes proliferation in cyclophosphamide-treated mice.Conclu-sion YPF solution can partially relieve the inhibition effect of Cyclophosphamide in immune function of C 57BL/6 mouse.