CAJ | 학술논문

目的检测系统性红斑狼疮（SLE）患者血浆白细胞介素（IL）-35水平，探讨其在SLE患者血浆中的变化及与疾病活动性的关系。方法采用酶联免疫吸附试验（ELISA）检测30例SLE患者及15名健康体检者血浆IL-35水平，比较其在各组中的差异及其与SLE患者部分临床指标的相关性，两组间比较用独立样本t检验，三组间比较采用方差分析，与临床资料的相关分析采用非参数的spearmen的相关分析。结果活动期SLE患者血浆IL-35水平（10.7±3.9）pg/ml低于健康对照组（55.2±15.6）pg/ml (P<0.01)及SLE稳定组（25.3±8.1）pg/ml (P<0.05)，稳定组亦低于健康对照组(P<0.05)。SLE患者血浆IL-35水平与抗核抗体（ANA）、抗dsDNA、红细胞沉降率（ESR）、24h尿蛋白定量、SLE活动指数（SLEDAI）呈负相关，与补体（C）3呈正相关。结论 SLE患者血浆IL-35水平降低，与疾病活动性相关，提示IL-35在SLE的进展中可能发挥重要作用，并可作为反映SLE活动性的指标。
목적검측계통성홍반랑창（SLE）환자혈장백세포개소（IL）-35수평，탐토기재SLE환자혈장중적변화급여질병활동성적관계。방법채용매련면역흡부시험（ELISA）검측30례SLE환자급15명건강체검자혈장IL-35수평，비교기재각조중적차이급기여SLE환자부분림상지표적상관성，량조간비교용독립양본t검험，삼조간비교채용방차분석，여림상자료적상관분석채용비삼수적spearmen적상관분석。결과활동기SLE환자혈장IL-35수평（10.7±3.9）pg/ml저우건강대조조（55.2±15.6）pg/ml (P<0.01)급SLE은정조（25.3±8.1）pg/ml (P<0.05)，은정조역저우건강대조조(P<0.05)。SLE환자혈장IL-35수평여항핵항체（ANA）、항dsDNA、홍세포침강솔（ESR）、24h뇨단백정량、SLE활동지수（SLEDAI）정부상관，여보체（C）3정정상관。결론 SLE환자혈장IL-35수평강저，여질병활동성상관，제시IL-35재SLE적진전중가능발휘중요작용，병가작위반영SLE활동성적지표。
Objective To investigate the serum IL-35 levels in patients with systemic lupus erytbematosus (SLE), explore the role of IL-35 in the pathogenesis of SLE and their relationship with disease activity. Methods 30 hospitalized SLE patients were recruited and 15 healthy volunteers were enrolled as normal controls, serum IL-35 levels in the peripheral blood of SLE patients and healthy controls were measured by enzyme-linked immunosorbent assay (ELISA). Independent sample t test was used for statistical analysis between two groups, analysis of variance (ANOVA) was applied for data analysis between three groups,non-parameterical Spearman’s analysis was used for correlation analysis. Results The serum IL-35 levels was decreased in active SLE patients [(10.7±3.9) pg/ml)] compared with inactive SLE patients [(25.3±8.1) pg/ml (P<0.05)] and healthy controls [(55.2±15.6) pg/ml) (P<0.01)], inactive SLE patients is lower than controls (P<0.05) , the serum IL-35 levels were negatively correlated with the titer of anti-dsDNA and ANA、SLEDAI、levels of 24-hour urinary protein and ESR, positively correlated with the levels of C3. Conclusion The serum IL-35 levels in SLE patients were elevated significantly and correlated with disease activity, these results demonstrate IL-35 may play an important role in the pathogenesis of SLE, the serum IL-35 levels are parameters for evaluation of SLE activity and severity.