中外健康文摘
中外健康文摘
중외건강문적
WORLD HEALTH DIGEST
2013年
22期
196-197
,共2页
余大为%侯言东(通讯作者)
餘大為%侯言東(通訊作者)
여대위%후언동(통신작자)
联合诱导法%S9制备%蛋白含量测定
聯閤誘導法%S9製備%蛋白含量測定
연합유도법%S9제비%단백함량측정
combination inducing method%S9 preparation%protein determination
目的测定联合诱导法和多氯联苯诱导法制备的大鼠肝S9的蛋白含量并对这两种方法进行比较。方法采用苯巴比妥与β—萘黄酮合并诱导所获S9作为诱导剂制备大鼠肝S9,并采用Bradford法测定所制备的S9蛋白含量。结果成功制得大鼠肝S9,并测定其蛋白含量为26.73 mg/ml。结论联合诱导法可以替代多氯联苯诱导法制备肝匀浆S9。
目的測定聯閤誘導法和多氯聯苯誘導法製備的大鼠肝S9的蛋白含量併對這兩種方法進行比較。方法採用苯巴比妥與β—萘黃酮閤併誘導所穫S9作為誘導劑製備大鼠肝S9,併採用Bradford法測定所製備的S9蛋白含量。結果成功製得大鼠肝S9,併測定其蛋白含量為26.73 mg/ml。結論聯閤誘導法可以替代多氯聯苯誘導法製備肝勻漿S9。
목적측정연합유도법화다록련분유도법제비적대서간S9적단백함량병대저량충방법진행비교。방법채용분파비타여β—내황동합병유도소획S9작위유도제제비대서간S9,병채용Bradford법측정소제비적S9단백함량。결과성공제득대서간S9,병측정기단백함량위26.73 mg/ml。결론연합유도법가이체대다록련분유도법제비간균장S9。
Objective: To determine the content of protein in rat liver S9 prepared by combination inducing method and PCB inducing method and compare the two methods. Methods: Rat liver S9 was prepared using S9 obtained by combination inducing method with phenobarbital and β-naphthoflavone as the inducer and the content of protein in the prepared S9 was determined by Bradford method. Results: Rat liver S9 was successfully prepared and the protein content was 26.73 mg/ml. Conclusion: Combination inducing method may supersede PCB inducing method for preparation of liver homogenate S9.
