CAJ | 학술논문

目的：探讨丙型肝炎病毒核心抗原（HCV-cAg）检测在丙型肝炎（丙肝）感染窗口期和免疫异常患者中的临床价值。方法2011年6月至2014年1月分别对丙肝病毒抗体（HCV-Ab）阳性标本153份（Ⅰ组），HCV-Ab阴性血液透析患者、吸毒患者和救助站“三无（无身份证、无工作、无住处）”人员的标本195份（Ⅱ组），HCV-Ab阴性的肿瘤科患者标本207份（Ⅲ组）和HCV-Ab阴性的健康对照标本71份（Ⅳ组），采用酶联免疫吸附试验法检测HCV-cAg；对HCV-cAg检测结果阳性标本进一步检测HCV RNA，对HCV-cAg和HCV-Ab均阳性的吸光度值（S/CO）结果进行比较分析。结果在Ⅰ、Ⅱ、Ⅲ、Ⅳ组中HCV-cAg的阳性率分别为29.4%（45/153）、5.6%（11/195）、3.4%（7/207）、1.4%（1/71）；HCV-cAg 与HCV RNA的符合率达95.3%；随着HCV-cAg水平的下降，HCV-Ab水平呈上升趋势。结论 HCV-cAg检测与HCV RNA符合率高，能防止窗口期和免疫异常丙肝患者的漏诊；HCV-cAg与HCV-Ab互为补充关系，二者联合检测能提高丙肝检出率。
목적：탐토병형간염병독핵심항원（HCV-cAg）검측재병형간염（병간）감염창구기화면역이상환자중적림상개치。방법2011년6월지2014년1월분별대병간병독항체（HCV-Ab）양성표본153빈（Ⅰ조），HCV-Ab음성혈액투석환자、흡독환자화구조참“삼무（무신빈증、무공작、무주처）”인원적표본195빈（Ⅱ조），HCV-Ab음성적종류과환자표본207빈（Ⅲ조）화HCV-Ab음성적건강대조표본71빈（Ⅳ조），채용매련면역흡부시험법검측HCV-cAg；대HCV-cAg검측결과양성표본진일보검측HCV RNA，대HCV-cAg화HCV-Ab균양성적흡광도치（S/CO）결과진행비교분석。결과재Ⅰ、Ⅱ、Ⅲ、Ⅳ조중HCV-cAg적양성솔분별위29.4%（45/153）、5.6%（11/195）、3.4%（7/207）、1.4%（1/71）；HCV-cAg 여HCV RNA적부합솔체95.3%；수착HCV-cAg수평적하강，HCV-Ab수평정상승추세。결론 HCV-cAg검측여HCV RNA부합솔고，능방지창구기화면역이상병간환자적루진；HCV-cAg여HCV-Ab호위보충관계，이자연합검측능제고병간검출솔。
Objective To investigate the clinical value of the hepatitis C virus core antigen (HCV-cAg) in window period and immune abnormalities of the patients with hepatitis C. Methods A total of 153 positive specimens of antibody to hepatitis C virus(HCV-Ab) (groupⅠ),195 specimens of“three non-personnel”(no ID,no job,no home) in salvation,drug users and hemodial-ysis patients with negative HCV-Ab(groupⅡ),207 specimens with negative HCV-Ab in Oncology Department(groupⅢ) and 71 specimens of healthy control with negative HCV-Ab(groupⅣ) were adopted from June 2011 to January 2014 by enzyme linked immunosorbent assay to detect the HCV-cAg. The HCV RNA in specimens with positive HCV-cAg were further detected,and the absorbance values(S/CO) of the specimen of positive HCV-cAg and HCV-Ab were compared and analyzed. Results The posi-tive rates of HCV-cAg in groupⅠ,Ⅱ,ⅢandⅣwere 29.4%(45/153),5.6%(11/195),3.4%(7/207) and 1.4%(1/71) respec-tively. The coincidence rate of HCV-cAg and HCV RNA reached to 95.3%. With the decline of HCV-cAg level,the level of HCV-Ab reflected upward trend. Conclusion The coincidence rate between HCV-cAg and HCV-RNA is high,HCV-cAg detection can prevent missed diagnosis in the window period and immune abnormalities of HCV patients. HCV-cAg and HCV-Ab show com-plementary relationship,the combined detection can greatly improve the detection rate of hepatitis C.