CAJ | 학술논문

目的探讨血管紧张素I在不同浓度和不同作用时间下对血管平滑肌细胞凋亡及凋亡调控基因表达的作用及心康对其影响。方法采用流式细胞技术测定在血管紧张素II不同浓度和不同作用时间下血管平滑肌细胞凋亡率及凋亡调控基因Fas、Bcl-2表达的变化和应用心康后对其影响。结果血管紧张素II对血管平滑肌细胞凋亡和凋亡调控基因表达有较明显的诱导作用；心康可抑制AngII作用，对细胞凋亡有一定的影响，同时可影响凋亡调控基因的表达。结论血管紧张素I具有一定促进血管平滑肌细胞凋亡作用和调节凋亡调控基因表达的作用，尤其在大剂量作用较明显；心康可明显抑制AngI对血管平滑肌细胞的作用。
목적탐토혈관긴장소I재불동농도화불동작용시간하대혈관평활기세포조망급조망조공기인표체적작용급심강대기영향。방법채용류식세포기술측정재혈관긴장소II불동농도화불동작용시간하혈관평활기세포조망솔급조망조공기인Fas、Bcl-2표체적변화화응용심강후대기영향。결과혈관긴장소II대혈관평활기세포조망화조망조공기인표체유교명현적유도작용；심강가억제AngII작용，대세포조망유일정적영향，동시가영향조망조공기인적표체。결론혈관긴장소I구유일정촉진혈관평활기세포조망작용화조절조망조공기인표체적작용，우기재대제량작용교명현；심강가명현억제AngI대혈관평활기세포적작용。
AIM:To study the effects of angiotensin II (AngII) in different concentrations and different action time on the ratio of apoptosis and expression of apoptosis genes in vascular smooth muscle cells and the influences of Xin-kang (XK) on it. METHODS: Flow cytometer was used to measure the apoptosis rate and the expression of apoptosis genes (Fas and Bcl-2) induced by AngII in different concentrations and different action time , and influenced by the drug.. RESULTS: The apoptosis rate and the expression of apoptosis genes were induced and increased by AngII with the increase of concentrations and action time compared with the control. The expression of Fas was increased and Bcl-2, decreased with the increase of the concentrations and action time of AngII after the uses of the drug compared with AngII group. CONCLUSION: The apoptosis rate and the expression of apoptosis genes may be induced and increased by angiotensin II with the increase of the concentrations and the action time. The use of drug may have the regulating effect on the apoptosis rate and the expression of apoptosis genes in vascular smooth muscle cells.