CAJ | 학술논문

目的观察大鼠大脑皮质γ-氨基丁酸A受体(GABAAR)及受体基因(GABAARα1mRNA)在脑缺血不同时段的表达及其动态变化的规律,探讨其在急性脑缺血中变化的意义.方法55只雄性Wistar大鼠随机分为对照组,脑缺血6 h组、12 h组、24 h组、3 d组、7 d组(均为手术组),假手术组；采用大鼠大脑中动脉栓塞(MCAO)模型,至规定时间点取大脑组织,用光学显微镜观察神经元损伤程度,用形态分析系统检测各组G A B A A R表达的阳性细胞数.用地高辛标记寡聚核苷酸探针原位杂交技术与计算机分析系统在光学显微镜下观察GABAARα1mRNA在大脑皮质阳性神经元的表达情况.结果各手术组GABAAR及GABAARα1mRNA表达均低于假手术组及对照组,与对照组相比, GABAAR在缺血6 h其表达既有降低,至12 h降至最低.GABAARα1mRNA表达在缺血24h开始下降,3d下降显著,7d恢复正常,而假手术组与对照组相比无统计学意义.结论 GABAAR及 GABAARα1mRNA在急性脑缺血时含量降低可能为脑缺血时脑损伤的重要原因.
목적관찰대서대뇌피질γ-안기정산A수체(GABAAR)급수체기인(GABAARα1mRNA)재뇌결혈불동시단적표체급기동태변화적규률,탐토기재급성뇌결혈중변화적의의.방법55지웅성Wistar대서수궤분위대조조,뇌결혈6 h조、12 h조、24 h조、3 d조、7 d조(균위수술조),가수술조；채용대서대뇌중동맥전새(MCAO)모형,지규정시간점취대뇌조직,용광학현미경관찰신경원손상정도,용형태분석계통검측각조G A B A A R표체적양성세포수.용지고신표기과취핵감산탐침원위잡교기술여계산궤분석계통재광학현미경하관찰GABAARα1mRNA재대뇌피질양성신경원적표체정황.결과각수술조GABAAR급GABAARα1mRNA표체균저우가수술조급대조조,여대조조상비, GABAAR재결혈6 h기표체기유강저,지12 h강지최저.GABAARα1mRNA표체재결혈24h개시하강,3d하강현저,7d회복정상,이가수술조여대조조상비무통계학의의.결론 GABAAR급 GABAARα1mRNA재급성뇌결혈시함량강저가능위뇌결혈시뇌손상적중요원인.
Objecive To observe the expression of GABAAR and GABAARα1mRNA and the dynamic regulation in the cortex of rats with focal cerebral ischemia in different phases and to investigate its significance in acute ischemia. Methods: Fifty-five male Wistar rats were randomly divided into 11 groups: The normal control, sham operation and ischemic operation group(subdivided into 6,12,24 hour and 3,7 days) by middle cerebral artery occlusion model. Brain specimens of the rats from each group were obtained to observe the damage of neurons with light microscope and to judge the positive expression of neurons in immunohistochemistry technique, and then to determine the positive cell number ex-pressed by GABAAR in each group with morphologic analysis system, and the changes of GABAARα1mRNA expression were observed under light microscope with digoxin labeled oligonucleotide probe in situhybridization method and computer aided image analysis. Results: Compared with the normal control group and the sham operation group, the expression of GABAAR and GABAARα1mRNA in each ischemia operation group was decreased. the expression of GABAAR was decreased after 6h and reached the bottom within 12 hours after ischemia. the expression of GABAARα1mRNA was decreased from 24h and was the lowest within 3d and recovered to normal in 7d after ischemia. However, there was no significant difference between the normal control group and sham operation group. Conclusion:It is suggested that the decreasing of content of GABAAR and GABAARα1mRNA after ischemia might be related with ischemia- injury.