中外健康文摘
中外健康文摘
중외건강문적
WORLD HEALTH DIGEST
2013年
6期
14-16
,共3页
梅毒抗体%血清学试验%方法比较
梅毒抗體%血清學試驗%方法比較
매독항체%혈청학시험%방법비교
Syphilis antibody%Serological test%Method comparison
目的了解梅毒快速血浆反应素试验(RPR)、梅毒甲苯胺红不加热血清试验(TRUST)、酶联免疫吸附试验(ELISA)和梅毒螺旋体抗体被动颗粒凝集试验(TPPA)的敏感性和特异性。方法采用RPR、TRUST、ELISA和TPPA同时对202份梅毒患者血清标本进行检测,以TPPA作为参考标准,分别统计RPR、TRUST和ELISA的敏感性和特异性。结果 RPR、TRUST、ELISA诊断梅毒的敏感性分别为84.16%、88.12%、98.51%;特异性均为96.67%;ELISA与TPPA检测阳性率差异无统计学意义,RPR、TRUST与TPPA法检测阳性率差异有统计学意义。结论 ELISA法具有较高的敏感性和特异性法,可用于临床筛查和梅毒感染的确证试验。
目的瞭解梅毒快速血漿反應素試驗(RPR)、梅毒甲苯胺紅不加熱血清試驗(TRUST)、酶聯免疫吸附試驗(ELISA)和梅毒螺鏇體抗體被動顆粒凝集試驗(TPPA)的敏感性和特異性。方法採用RPR、TRUST、ELISA和TPPA同時對202份梅毒患者血清標本進行檢測,以TPPA作為參攷標準,分彆統計RPR、TRUST和ELISA的敏感性和特異性。結果 RPR、TRUST、ELISA診斷梅毒的敏感性分彆為84.16%、88.12%、98.51%;特異性均為96.67%;ELISA與TPPA檢測暘性率差異無統計學意義,RPR、TRUST與TPPA法檢測暘性率差異有統計學意義。結論 ELISA法具有較高的敏感性和特異性法,可用于臨床篩查和梅毒感染的確證試驗。
목적료해매독쾌속혈장반응소시험(RPR)、매독갑분알홍불가열혈청시험(TRUST)、매련면역흡부시험(ELISA)화매독라선체항체피동과립응집시험(TPPA)적민감성화특이성。방법채용RPR、TRUST、ELISA화TPPA동시대202빈매독환자혈청표본진행검측,이TPPA작위삼고표준,분별통계RPR、TRUST화ELISA적민감성화특이성。결과 RPR、TRUST、ELISA진단매독적민감성분별위84.16%、88.12%、98.51%;특이성균위96.67%;ELISA여TPPA검측양성솔차이무통계학의의,RPR、TRUST여TPPA법검측양성솔차이유통계학의의。결론 ELISA법구유교고적민감성화특이성법,가용우림상사사화매독감염적학증시험。
Objective: Knowledge about syphilis rapid plasma reagin test (RPR), syphilis toluidine red unheated serum test (TRUST), enzyme linked immunosorbent assay (ELISA) and treponemal antibody passive particle agglutination test (TPPA) sensitivity and specificity. Methods:Using RPR, TRUST, ELISA and TPPA simultaneously on 202 patients with syphilis sera, taking TPPA as the reference standard, RPR, TRUST and ELISA were calculated the sensitivity and specificity of. Results:RPR, TRUST, ELISA diagnosis of syphilis in a sensitivity of 84.16%, 88.12%, 98.51%; specificity was 96.67%;ELISA and TPPA positive rate was no significant difference, RPR, TRUST and TPPA method for detecting positive rate had a significant difference. Conclusion:The ELISA method has high sensitivity and specificity of the method, can be used for clinical screening and confirmatory test for syphilis infection.