CAJ | 학술논문

建立高效液相色谱-质谱联用法(LC-MS/MS)测定大鼠灌胃给药的生物利用度.大鼠随机分为2组,分别尾静脉和灌胃给予100 mg·kg-1木通皂苷D,于不同时间点眼眶取血,用 LC-MS/MS法检测血浆中药物质量浓度,计算大鼠灌胃给药的生物利用度.结果表明：木通皂苷D线性范围为10~1000 ng·mL-1,最低定量限为10 ng·mL-1.准确度与精密度试验结果显示方法日间、日内变异均小于15%,相对偏差为-2.8%~4.6%,低、中、高3个质量浓度提取回收率为95.3%~108.1%.静脉组和口服组的药时曲线下面积 AUC0-INF分别为(231725.98±46527.21)和(383.63±54.62) ng·h·mL-1,灌胃给药的绝对生物利用度为0.13%.表明木通皂苷D大鼠灌胃给药生物利用度很低.
건립고효액상색보-질보련용법(LC-MS/MS)측정대서관위급약적생물이용도.대서수궤분위2조,분별미정맥화관위급여100 mg·kg-1목통조감D,우불동시간점안광취혈,용 LC-MS/MS법검측혈장중약물질량농도,계산대서관위급약적생물이용도.결과표명：목통조감D선성범위위10~1000 ng·mL-1,최저정량한위10 ng·mL-1.준학도여정밀도시험결과현시방법일간、일내변이균소우15%,상대편차위-2.8%~4.6%,저、중、고3개질량농도제취회수솔위95.3%~108.1%.정맥조화구복조적약시곡선하면적 AUC0-INF분별위(231725.98±46527.21)화(383.63±54.62) ng·h·mL-1,관위급약적절대생물이용도위0.13%.표명목통조감D대서관위급약생물이용도흔저.
A LC-MS/MS method was used to determine oral bioavailability of akebia saponin D in rat.SD rats were randomly divided into two groups.Dose of 100 mg·kg-1 akebia saponin D was given by stomach tube or by caudal vein inj ection.Blood samples were collected at different time intervals after administration through orbit vein.Plasma drug concentration was detected by LC-MS/MS,oral bioavailability was then calculated. The LC-MS/MS assay calibration curve was linear over the range of 10~1 000 ng·mL-1 .Intra-and inter-day variations were both less than 1 5%.Relative deviation was from -2.8%~4.6%.Recoveries of akebia saponin D were from 95.3%~108.1%.AUC0-INF in injection group and oral group were (231 725.98±46 527.21)and (383.63±54.62)ng·h·mL-1 respectively.Absolute bioavailability for intragastric administration was 0.13%. Bioavailability of akebia saponin D by intragastric administration was very low.