北京师范大学学报(自然科学版)
北京師範大學學報(自然科學版)
북경사범대학학보(자연과학판)
JOURNAL OF BEIJING NORMAL UNIVERSITY
2014年
1期
58-61
,共4页
白腰文鸟%高级发声中枢%性双态性%增殖
白腰文鳥%高級髮聲中樞%性雙態性%增殖
백요문조%고급발성중추%성쌍태성%증식
Lonchura striatu%HVC%sexual dimorphism%cellular proliferation
为了更有针对性地研究鸣禽高级发声中枢(H VC)性双态性的形成过程,本文以白腰文鸟(Lonchura striata )为实验对象,首先通过vimentin免疫组织化学分析端脑 HVC区域放射胶质细胞纤维的起源与走向,推断出幼鸟孵化后的 H VC正背侧VZ区为 H VC细胞的主要发源位点;在此基础上,对15 d幼鸟经由胸大肌注射3 H-胸腺嘧啶以标记端脑新生细胞,通过3 H放射性自显影技术比较雌、雄 H VC 干细胞的增殖能力,充分证明了 H VC 细胞发源点的神经干细胞细胞增殖能力具有显著的性别差异。
為瞭更有針對性地研究鳴禽高級髮聲中樞(H VC)性雙態性的形成過程,本文以白腰文鳥(Lonchura striata )為實驗對象,首先通過vimentin免疫組織化學分析耑腦 HVC區域放射膠質細胞纖維的起源與走嚮,推斷齣幼鳥孵化後的 H VC正揹側VZ區為 H VC細胞的主要髮源位點;在此基礎上,對15 d幼鳥經由胸大肌註射3 H-胸腺嘧啶以標記耑腦新生細胞,通過3 H放射性自顯影技術比較雌、雄 H VC 榦細胞的增殖能力,充分證明瞭 H VC 細胞髮源點的神經榦細胞細胞增殖能力具有顯著的性彆差異。
위료경유침대성지연구명금고급발성중추(H VC)성쌍태성적형성과정,본문이백요문조(Lonchura striata )위실험대상,수선통과vimentin면역조직화학분석단뇌 HVC구역방사효질세포섬유적기원여주향,추단출유조부화후적 H VC정배측VZ구위 H VC세포적주요발원위점;재차기출상,대15 d유조경유흉대기주사3 H-흉선밀정이표기단뇌신생세포,통과3 H방사성자현영기술비교자、웅 H VC 간세포적증식능력,충분증명료 H VC 세포발원점적신경간세포세포증식능력구유현저적성별차이。
To analyze mechanism of sexually dimorphic development of high vocal center (HVC)in songbird,it was confirmed that ventricular zone (VZ)dorsal to HVC was an important source of HVC cells, and their migratory route was identified by the distribution of radial glial cells in telencephalon in Lonchura striatu with vimentin antibody immunohistology;Male and female Lonchura striatu 1 5 d post-hatching received intramuscular inj ection of 3 H-thymidine,the number of newborn cells in VZ dorsal to HVC was counted by 3 H-thymidine autoradiography.Such data proved remarkable sex difference in cell proliferation in the origin of HVC cells.