中外健康文摘
中外健康文摘
중외건강문적
WORLD HEALTH DIGEST
2014年
3期
34-36
,共3页
Claudin-7%基因敲除%裸鼠%结肠癌%肝转移
Claudin-7%基因敲除%裸鼠%結腸癌%肝轉移
Claudin-7%기인고제%라서%결장암%간전이
Claudin-7%Gene knockdown%Nude mice%Colon cancer%Liver metastasis
目的：研究Claudin-7基因敲除对裸鼠结肠癌肝转移模型中的致癌作用及其前临床意义。方法：采用Claudin-7基因敲除及基因重组质粒pcDNA6-Claudin-7基因敲除转染的HT29结肠癌细胞建立裸鼠结肠癌肝转移模型，随机分为2组，即Claudin-7敲除组和对照组。制模60天后比较两组间转移瘤形成情况，并采用免疫组织化学检测两组肝转移组织的Claudin-7表达程度。结果：Claudin-7敲除组肝质量、肝转移评分、淋巴结转移发生率、血性腹水成率、腹膜种植转移发生率、远处转移发生率明显高于对照组，两组间差异有统计学意义(p<0.05)。肝转移的组织病理学检测中，发现Claudin-7表达在两组间有显著差异，并有统计学意义(p<0.05)。生命表证实，Claudin-7敲除组生存率明显低于对照组，差异有统计学意义(p<0.01)。结论：Claudin-7基因敲除可促进裸鼠结肠癌肝转移的形成，况且Claudin-7表达可作为肿瘤转移的评价指标。
目的：研究Claudin-7基因敲除對裸鼠結腸癌肝轉移模型中的緻癌作用及其前臨床意義。方法：採用Claudin-7基因敲除及基因重組質粒pcDNA6-Claudin-7基因敲除轉染的HT29結腸癌細胞建立裸鼠結腸癌肝轉移模型，隨機分為2組，即Claudin-7敲除組和對照組。製模60天後比較兩組間轉移瘤形成情況，併採用免疫組織化學檢測兩組肝轉移組織的Claudin-7錶達程度。結果：Claudin-7敲除組肝質量、肝轉移評分、淋巴結轉移髮生率、血性腹水成率、腹膜種植轉移髮生率、遠處轉移髮生率明顯高于對照組，兩組間差異有統計學意義(p<0.05)。肝轉移的組織病理學檢測中，髮現Claudin-7錶達在兩組間有顯著差異，併有統計學意義(p<0.05)。生命錶證實，Claudin-7敲除組生存率明顯低于對照組，差異有統計學意義(p<0.01)。結論：Claudin-7基因敲除可促進裸鼠結腸癌肝轉移的形成，況且Claudin-7錶達可作為腫瘤轉移的評價指標。
목적：연구Claudin-7기인고제대라서결장암간전이모형중적치암작용급기전림상의의。방법：채용Claudin-7기인고제급기인중조질립pcDNA6-Claudin-7기인고제전염적HT29결장암세포건립라서결장암간전이모형，수궤분위2조，즉Claudin-7고제조화대조조。제모60천후비교량조간전이류형성정황，병채용면역조직화학검측량조간전이조직적Claudin-7표체정도。결과：Claudin-7고제조간질량、간전이평분、림파결전이발생솔、혈성복수성솔、복막충식전이발생솔、원처전이발생솔명현고우대조조，량조간차이유통계학의의(p<0.05)。간전이적조직병이학검측중，발현Claudin-7표체재량조간유현저차이，병유통계학의의(p<0.05)。생명표증실，Claudin-7고제조생존솔명현저우대조조，차이유통계학의의(p<0.01)。결론：Claudin-7기인고제가촉진라서결장암간전이적형성，황차Claudin-7표체가작위종류전이적평개지표。
Objective: To study the carcinogenic effects of Claudin-7 gene knockdown on the liver metastasis of colon cancer (LMCC) nude mice model(NMM) and its preclinical research. Methods: After 60 days of the spleen injection, HT29 colon cancer cells transfected by recombinant clone vector pcDNA6-Claudin-7 gene knockdown were used to establish the LMCC-NMM. The tumor bearing nude mice were randomized into 2 groups, Claudin-7 knockdown group and Scramble control group. In addition, Claudin-7 knockdown group was cancer cells injected into spleen to study group and control group, evaluating metastatic tumor formation, liver metastasis tissues were investigated expression of Claudin-7 protein via immunohistochemistry. Results: The liver weight, liver metastasis scores, lymph node metastasis rate, bloody ascites rate, peritoneal seeding rate, and distant metastasis rate of Claudin-7 knockdown group were significantly greater than that in the control group (p<0.05). Expression of Claudin-7 protein was marked histopathological difference of liver metastasis tissues between Claudin-7 knockdown group and control group,and difference was statistical y significant (p<0.05). Live tables showed that Claudin-7 knockdown group is inversely correlated with cumulative survival of LMCC-NMM (p<0.01). Conclusion: Claudin-7 gene knockdown may be promote the formation of LMCC-NMM, and expression of Claudin-7 as an evaluating index for tumor metastasis.
