山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
10期
7-9,13
,共4页
其曼古丽·吐尔洪%布威海丽且木·阿巴拜科日%祖丽比亚·司马义%买热艳木·艾尔肯%阿达莱提·麦麦提%依米提·热合曼
其曼古麗·吐爾洪%佈威海麗且木·阿巴拜科日%祖麗比亞·司馬義%買熱豔木·艾爾肯%阿達萊提·麥麥提%依米提·熱閤曼
기만고려·토이홍%포위해려차목·아파배과일%조려비아·사마의%매열염목·애이긍%아체래제·맥맥제%의미제·열합만
结肠癌%蜂胶黄酮%磷酸二酯酶4D%DNA损伤诱导基因G
結腸癌%蜂膠黃酮%燐痠二酯酶4D%DNA損傷誘導基因G
결장암%봉효황동%린산이지매4D%DNA손상유도기인G
colonic carcinoma%propolis flavonoids%phosphodiesterase 4D%DNA damage-inducible gene G
目的：探讨蜂胶黄酮（PB3A）对人结肠癌SW480细胞生长的抑制作用及机制。方法将培养的人结肠癌SW480细胞分为PB3A组及对照组。 PB3A组予100μg/mL PB3A干预，对照组不干预。干预后两组均培养24 h，倒置显微镜观察细胞形态学变化；采用实时荧光定量PCR法和蛋白免疫印迹法检测细胞磷酸二酯酶4D（PDE4D）、生长阻滞和DNA损伤诱导基因G（ Gadd45G） mRNA和蛋白表达。结果干预后倒置显微镜下可见PB3A组细胞缩小、皱缩、折光性减弱，细胞内出现颗粒状物质；对照组无明显变化。 PB3A组PDE4D mRNA及蛋白表达水平均低于对照组， Gadd45G mRNA及蛋白水平表达均高于对照组（P均＜0．01）。结论 PB3A能抑制SW480细胞生长，诱导其凋亡；其作用机制可能为下调PDE4D mRNA和蛋白表达，上调Gadd45G mRNA和蛋白表达。
目的：探討蜂膠黃酮（PB3A）對人結腸癌SW480細胞生長的抑製作用及機製。方法將培養的人結腸癌SW480細胞分為PB3A組及對照組。 PB3A組予100μg/mL PB3A榦預，對照組不榦預。榦預後兩組均培養24 h，倒置顯微鏡觀察細胞形態學變化；採用實時熒光定量PCR法和蛋白免疫印跡法檢測細胞燐痠二酯酶4D（PDE4D）、生長阻滯和DNA損傷誘導基因G（ Gadd45G） mRNA和蛋白錶達。結果榦預後倒置顯微鏡下可見PB3A組細胞縮小、皺縮、摺光性減弱，細胞內齣現顆粒狀物質；對照組無明顯變化。 PB3A組PDE4D mRNA及蛋白錶達水平均低于對照組， Gadd45G mRNA及蛋白水平錶達均高于對照組（P均＜0．01）。結論 PB3A能抑製SW480細胞生長，誘導其凋亡；其作用機製可能為下調PDE4D mRNA和蛋白錶達，上調Gadd45G mRNA和蛋白錶達。
목적：탐토봉효황동（PB3A）대인결장암SW480세포생장적억제작용급궤제。방법장배양적인결장암SW480세포분위PB3A조급대조조。 PB3A조여100μg/mL PB3A간예，대조조불간예。간예후량조균배양24 h，도치현미경관찰세포형태학변화；채용실시형광정량PCR법화단백면역인적법검측세포린산이지매4D（PDE4D）、생장조체화DNA손상유도기인G（ Gadd45G） mRNA화단백표체。결과간예후도치현미경하가견PB3A조세포축소、추축、절광성감약，세포내출현과립상물질；대조조무명현변화。 PB3A조PDE4D mRNA급단백표체수평균저우대조조， Gadd45G mRNA급단백수평표체균고우대조조（P균＜0．01）。결론 PB3A능억제SW480세포생장，유도기조망；기작용궤제가능위하조PDE4D mRNA화단백표체，상조Gadd45G mRNA화단백표체。
Objective To investigate the inhibitory effect of propolis flavonoids pinobanksin-3-acetate (PB3A) on the human colon cancer cell line SW 480 and to explore the underlying mechanism .Methods The cultivated human colon cancer cell line SW480 was randomly divided into the PB3A group and the control group .Cells in the PB3A group were in-tervened by 100 μg/mL PB3A, and the control group without intervention .We observed the cellular morphology by invert microscope after incubation for 24 h of the two groups .Real-time fluorescence quantitative polymerase chain reaction and Western blotting were performed to measure the expression of phosphodiesterase 4D (PDE4D) and growth arrest and DNA damage-inducible gene G (Gadd 45G) at mRNA and protein levels.Results After treated with 100 μg/mL PB3A, the colon cancer cells became shrunk , smaller, refraction decreased with granular material appeared intra-celluler in the PB3A group.In comparison with those in the control group , the mRNA and protein expression of PDE4D was down-regulated, while the mRNA and protein expression of Gadd 45G was up-regulated (all P<0.01).Conclusions PB3A inhibited the proliferation of SW480 cells and induced the apoptosis .Its possible mechanisms may be related to down-regulation of PDE4D expression and up-regulation of Gadd45G expression.
