山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
15期
4-7
,共4页
李友%廖锋%崔理立%马国达%李克深
李友%廖鋒%崔理立%馬國達%李剋深
리우%료봉%최리립%마국체%리극심
去整合素金属蛋白酶10基因%腔隙性脑梗死%基因多态性
去整閤素金屬蛋白酶10基因%腔隙性腦梗死%基因多態性
거정합소금속단백매10기인%강극성뇌경사%기인다태성
a disintegrin and metalloproteinase 10 gene%lacunar infarction%gene polymorphism
目的：观察腔隙性脑梗死患者去整合素金属蛋白酶（ADAM）10基因启动子区多态性变化，分析此多态性变化与腔隙性脑梗死发病风险的关系。方法研究对象为173例腔隙性脑梗死患者（梗死组）和297例非脑血管病体检者（对照组），采用Snapshot分型技术检测ADAM10基因启动子区rs653765和rs514049位点多态性，采用B型彩色多普勒超声仪测量颈动脉内膜中层厚度（ CIMT）；比较两组位点多态性及其与梗死组性别、年龄、CIMT的关系。结果梗死组ADAM10基因rs653765位点CC基因型频率显著低于对照组（ P＝0．0080）、隐性模型CT＋TT基因型频率显著高于对照组（OR＝0．54，95％CI为0．36～0．81，P＝0．0029），T等位基因频率显著高于对照组（OR＝1．95，95％CI为1．37～2．79，P＝0．0002）；两组ADAM10基因rs514049位点的基因型频率和等位基因频率无显著差异（P＞0．05）。携带ADAM10基因rs653765位点T等位基因的男性发生腔隙性脑梗死的风险显著升高（ OR＝1．51，95％CI为1．00～2．28，P＝0．048），而≥70岁人群中携带CT基因型或T等位基因者发生腔隙性脑梗死的风险显著升高（OR＝1．69，95％CI为1．00～2．85， P＝0．046）；ADAM10基因rs653765和rs514049位点的多态性与腔隙性脑梗死患者CI-MT无明显相关。结论 ADAM10基因rs653765位点T等位基因可能与腔隙性脑梗死的发病有关。
目的：觀察腔隙性腦梗死患者去整閤素金屬蛋白酶（ADAM）10基因啟動子區多態性變化，分析此多態性變化與腔隙性腦梗死髮病風險的關繫。方法研究對象為173例腔隙性腦梗死患者（梗死組）和297例非腦血管病體檢者（對照組），採用Snapshot分型技術檢測ADAM10基因啟動子區rs653765和rs514049位點多態性，採用B型綵色多普勒超聲儀測量頸動脈內膜中層厚度（ CIMT）；比較兩組位點多態性及其與梗死組性彆、年齡、CIMT的關繫。結果梗死組ADAM10基因rs653765位點CC基因型頻率顯著低于對照組（ P＝0．0080）、隱性模型CT＋TT基因型頻率顯著高于對照組（OR＝0．54，95％CI為0．36～0．81，P＝0．0029），T等位基因頻率顯著高于對照組（OR＝1．95，95％CI為1．37～2．79，P＝0．0002）；兩組ADAM10基因rs514049位點的基因型頻率和等位基因頻率無顯著差異（P＞0．05）。攜帶ADAM10基因rs653765位點T等位基因的男性髮生腔隙性腦梗死的風險顯著升高（ OR＝1．51，95％CI為1．00～2．28，P＝0．048），而≥70歲人群中攜帶CT基因型或T等位基因者髮生腔隙性腦梗死的風險顯著升高（OR＝1．69，95％CI為1．00～2．85， P＝0．046）；ADAM10基因rs653765和rs514049位點的多態性與腔隙性腦梗死患者CI-MT無明顯相關。結論 ADAM10基因rs653765位點T等位基因可能與腔隙性腦梗死的髮病有關。
목적：관찰강극성뇌경사환자거정합소금속단백매（ADAM）10기인계동자구다태성변화，분석차다태성변화여강극성뇌경사발병풍험적관계。방법연구대상위173례강극성뇌경사환자（경사조）화297례비뇌혈관병체검자（대조조），채용Snapshot분형기술검측ADAM10기인계동자구rs653765화rs514049위점다태성，채용B형채색다보륵초성의측량경동맥내막중층후도（ CIMT）；비교량조위점다태성급기여경사조성별、년령、CIMT적관계。결과경사조ADAM10기인rs653765위점CC기인형빈솔현저저우대조조（ P＝0．0080）、은성모형CT＋TT기인형빈솔현저고우대조조（OR＝0．54，95％CI위0．36～0．81，P＝0．0029），T등위기인빈솔현저고우대조조（OR＝1．95，95％CI위1．37～2．79，P＝0．0002）；량조ADAM10기인rs514049위점적기인형빈솔화등위기인빈솔무현저차이（P＞0．05）。휴대ADAM10기인rs653765위점T등위기인적남성발생강극성뇌경사적풍험현저승고（ OR＝1．51，95％CI위1．00～2．28，P＝0．048），이≥70세인군중휴대CT기인형혹T등위기인자발생강극성뇌경사적풍험현저승고（OR＝1．69，95％CI위1．00～2．85， P＝0．046）；ADAM10기인rs653765화rs514049위점적다태성여강극성뇌경사환자CI-MT무명현상관。결론 ADAM10기인rs653765위점T등위기인가능여강극성뇌경사적발병유관。
Objective To observe the changes of a disintegrin and metalloproteinase 10(ADAM10) promoter polymor-phisms and to investigate their correlations with the risk of lacunar infarction (LI).Methods Totally 173 patients (infarction group) with LI and 297 controls (control group) from the Department of Neurology of the Affiliated Hospital of Guangdong Medi-cal College were selected.Multiplex Snapshot was used to determine the genotype and allele frequencies of rs653765 and rs514049 polymorphisms of the ADAM10 gene.B-color Doppler ultrasonography was used to determine the carotid artery intima-media thickness (CIMT).The relationships between polymorphisms of the two sites and sex, age, CIMT in the infarction group were analyzed.Results The frequency of the ADAM10 rs653765 CC genotype carriers was significantly lower in the infarction group as compared with that of the control group (P=0.008 0), but in a recessive model, the frequency of CT+TT genotype was significantly higher (OR=0.54, 95%CI 0.36-0.81, P=0.002 9), and the ADAM10 rs653765 T allele was also signifi-cantly higher (OR=1.95, 95%CI 1.37-2.79, P=0.000 2).The genotype and allele frequencies of ADAM10 rs514049 had no significantly difference between the infarction group and the control group (P>0.05).Further stratification analysis on gender and age revealed that men carrying the ADAM10 rs653765 T allele conferred a higher risk of LI ( OR=1.51, 95%CI 1.00-2.28, P=0.048), and the individuals over 70 years old with the ADAM10 rs653765 CT genotype or T allele might run a higher risk of LI (OR=1.69, 95%CI 1.00-2.85, P=0.046).The genotype and allele frequencies of rs514049 were not statistically associated with LI, even after taking into account the patients'gender and age.Conclusion The present study suggests that the ADAM10 rs653765 T allele may be associated with the etiology of lacunar infarction.
