CAJ | 학술논문

目的：观察血管内皮生长因子（ VEGF）-A和VEGF-C在星形细胞肿瘤组织中的表达变化，分析两指标间及与肿瘤血管生成的关系。方法留取93例星形细胞肿瘤患者肿瘤组织及9例接受高血压脑出血开颅手术患者的正常脑组织，用免疫组织化学（SP）方法检测VEGF-A、VEGF-C蛋白表达和微血管密度（MVD），RT-PCR法检测VEGF-A、VEGF-C mRNA表达，对两VEGF间及与肿瘤WHO分级、MVD的关系进行统计学分析。结果星形细胞肿瘤组织中VEGF-A、VEGF-C蛋白阳性表达率及MVD均显著高于正常脑组织，且均随肿瘤恶性程度增高而增强（P均＜0．01）；星形细胞肿瘤组织中VEGF-A、VEGF-C蛋白表达及两者与MVD均呈显著相关（P均＜0．01）。星形细胞肿瘤组织中VEGF-A和VEGF-C mRNA表达均显著高于正常脑组织，且均随肿瘤恶性程度增高而增强（ P均＜0．05）；肿瘤组织中VEGF-A和VEGF-C mRNA表达呈显著相关（P均＜0．05）。结论 VEGF-A、VEGF-C在星形细胞肿瘤组织中的表达上调并与肿瘤恶性程度有关，两者间及与肿瘤血管生成均关系密切。
목적：관찰혈관내피생장인자（ VEGF）-A화VEGF-C재성형세포종류조직중적표체변화，분석량지표간급여종류혈관생성적관계。방법류취93례성형세포종류환자종류조직급9례접수고혈압뇌출혈개로수술환자적정상뇌조직，용면역조직화학（SP）방법검측VEGF-A、VEGF-C단백표체화미혈관밀도（MVD），RT-PCR법검측VEGF-A、VEGF-C mRNA표체，대량VEGF간급여종류WHO분급、MVD적관계진행통계학분석。결과성형세포종류조직중VEGF-A、VEGF-C단백양성표체솔급MVD균현저고우정상뇌조직，차균수종류악성정도증고이증강（P균＜0．01）；성형세포종류조직중VEGF-A、VEGF-C단백표체급량자여MVD균정현저상관（P균＜0．01）。성형세포종류조직중VEGF-A화VEGF-C mRNA표체균현저고우정상뇌조직，차균수종류악성정도증고이증강（ P균＜0．05）；종류조직중VEGF-A화VEGF-C mRNA표체정현저상관（P균＜0．05）。결론 VEGF-A、VEGF-C재성형세포종류조직중적표체상조병여종류악성정도유관，량자간급여종류혈관생성균관계밀절。
Objective To observe the expression changes of vascular endothelial growth factor-A (VEGF-A) and vascular endothelial growth factor-C (VEGF-C) in low-grade and high-grade astrocytic gliomas and their correlation with angiogenesis, which will provide the basis for target therapy and prognosis of astrocytomas .Methods Ninety-three astrocytic glioma samples and 9 normal brain tissues from spontaneous intracranial hemorrhage were obtained .The protein expression and microvessel den-sity (MVD) of VEGF-A and VEGF-C was examined with immunohistochemical staining method (S-P).The mRNA expression of VEGF-A and VEGF-C was examined with fluorescent quantitative polymerase chain reaction (RT-PCR).Statistical analysis was employed to determine the reciprocal correlation between VEGF-A and VEGF-C, and their correlation with WHO grades and MVD.Results The positive expression rates of VEGF-A, VEGF-C, and MVD in the astrocytic tumor tissues were significantly higher than those of the normal brain tissues, and the degree of expression was in accordance with the malignance of tumors (all P<0.01).The expression of VEGF-A, VEGF-C protein was correlated with MVD (all P<0.01).Meanwhile, the expression of VEGF-A, VEGF-C mRNA was higher than that of the normal tissues and the degree of expression was in accordance with the ma-lignance of tumors (all P<0.05), and the VEGF-A mRNA was significantly correlated with VEGF-C mRNA (all P<0.05). Conclusion The expression of VEGF-A and VEGF-C is up-regulated and is correlated with the malignancy of astrocytic tumor, which shows a close correlation between each other as well as their correlation with tumor angiogenesis .