现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2014年
10期
1449-1451
,共3页
心房颤动%微RNAs%心房重构
心房顫動%微RNAs%心房重構
심방전동%미RNAs%심방중구
Atrial Fibrillation%MicroRNAs%Atrial remodeling
目的：研究和分析microRNA（miRNA）在心房颤动的心房重构过程中的作用。方法选取于2012年9月至2013年11月在该院接受治疗的心房颤动患者198例作为研究对象，依据患者实际病况分成心房颤动组（104例）和窦性心律（窦律）组（94例），均行外科开胸术治疗，术中取样两组患者右心耳组织进行检测和分析，记录两组患者心房组织中miRNA的表达量和Cx43mRNA表达量，并采取苏木精-伊红染色观察患者心房组织形态的变化情况。结果心房颤动患者心房组织形态学发生明显变化，主要表现为心房细胞异常增大，肌纤维异常增粗且断裂，同时表现出大小不均、排列紊乱、细胞核增大、间质纤维化明显等特点。心房颤动组miRNA在心房组织中的表达量[（0.6149±0.1757）×103]明显低于窦律组[（2.2986±0.3906）×103]，差异有统计学意义（t=39.743，P<0.01）；但两组Cx43mRNA表达量比较，差异无统计学意义（t=1.224，P>0.05）；心房颤动组Cx43蛋白表达量[（0.5762±0.2010）×103]显著低于窦律组[（1.1450±0.3739）×103]，差异有统计学意义（t=13.507，P<0.01）。结论心房颤动患者心房重构明显；同时该类患者心房组织中miRNA的表达量明显下降，致使其相关的靶基因Cx43蛋白的表达量也随之下调，二者呈非负相关，说明miRNA并不能直接通过对Cx43的表达调控而参与到心房重构中。
目的：研究和分析microRNA（miRNA）在心房顫動的心房重構過程中的作用。方法選取于2012年9月至2013年11月在該院接受治療的心房顫動患者198例作為研究對象，依據患者實際病況分成心房顫動組（104例）和竇性心律（竇律）組（94例），均行外科開胸術治療，術中取樣兩組患者右心耳組織進行檢測和分析，記錄兩組患者心房組織中miRNA的錶達量和Cx43mRNA錶達量，併採取囌木精-伊紅染色觀察患者心房組織形態的變化情況。結果心房顫動患者心房組織形態學髮生明顯變化，主要錶現為心房細胞異常增大，肌纖維異常增粗且斷裂，同時錶現齣大小不均、排列紊亂、細胞覈增大、間質纖維化明顯等特點。心房顫動組miRNA在心房組織中的錶達量[（0.6149±0.1757）×103]明顯低于竇律組[（2.2986±0.3906）×103]，差異有統計學意義（t=39.743，P<0.01）；但兩組Cx43mRNA錶達量比較，差異無統計學意義（t=1.224，P>0.05）；心房顫動組Cx43蛋白錶達量[（0.5762±0.2010）×103]顯著低于竇律組[（1.1450±0.3739）×103]，差異有統計學意義（t=13.507，P<0.01）。結論心房顫動患者心房重構明顯；同時該類患者心房組織中miRNA的錶達量明顯下降，緻使其相關的靶基因Cx43蛋白的錶達量也隨之下調，二者呈非負相關，說明miRNA併不能直接通過對Cx43的錶達調控而參與到心房重構中。
목적：연구화분석microRNA（miRNA）재심방전동적심방중구과정중적작용。방법선취우2012년9월지2013년11월재해원접수치료적심방전동환자198례작위연구대상，의거환자실제병황분성심방전동조（104례）화두성심률（두률）조（94례），균행외과개흉술치료，술중취양량조환자우심이조직진행검측화분석，기록량조환자심방조직중miRNA적표체량화Cx43mRNA표체량，병채취소목정-이홍염색관찰환자심방조직형태적변화정황。결과심방전동환자심방조직형태학발생명현변화，주요표현위심방세포이상증대，기섬유이상증조차단렬，동시표현출대소불균、배렬문란、세포핵증대、간질섬유화명현등특점。심방전동조miRNA재심방조직중적표체량[（0.6149±0.1757）×103]명현저우두률조[（2.2986±0.3906）×103]，차이유통계학의의（t=39.743，P<0.01）；단량조Cx43mRNA표체량비교，차이무통계학의의（t=1.224，P>0.05）；심방전동조Cx43단백표체량[（0.5762±0.2010）×103]현저저우두률조[（1.1450±0.3739）×103]，차이유통계학의의（t=13.507，P<0.01）。결론심방전동환자심방중구명현；동시해류환자심방조직중miRNA적표체량명현하강，치사기상관적파기인Cx43단백적표체량야수지하조，이자정비부상관，설명miRNA병불능직접통과대Cx43적표체조공이삼여도심방중구중。
Objective To study and analyze the effect of microRNA on atrial structural remodeling in patients with atrial fibrillation(AF). Methods Totally 198 patients with AF,who received treatment from September 2012 to November 2013,were selected and divided into AF group(n=104) and sinus rhythm(SR) group(n=94) according to patients′actual condition. All the patients were with thoracotomy. The right auricle of the heart tissue in the two groups was sampled during the surgery for detection and analysis to record the expression quantity of microRNA and Cx43mRNA. Hematoxylin-eosin(HE) staining was adopted to ob-serve changes of atrial tissue morphology. Results Atrial tissue histomorphology of the patients with AF changed obviously , mainly on abnormal increase of atrial cell,abnormal enlargement and rupture of muscle fiber,uneven size,disordered arrange-ment,increase of neucleus,interstitial fibrosis and so on. The expression quantity of microRNA atrial tissue in AF group[(0.614 9±0.175 7)×103] was obviously lower than that in SR group[(2.298 6±0.390 6)×103] with statistically significant difference(t=39.743,P<0.01). The expression quantity of Cx43mRNA had no statistically significant difference between the two groups (t=1.224, P>0.05). The expression quantity of Cx43 protein in AF group[(0.576 2±0.201 0)×103] was remarkable lower than that in SR group [(1.145 0±0.373 9)×103],and the difference was statistically significant(t=13.507,P<0.01). Conclusion Atrial remodeling of the patients with AF is significant,at the same time,the expression quantity of microRNA in atrial tissue decreases dramatically, which resulting in the down-regulation of expression quantity of Cx43 protein related to target gene ,and the microRNA and Cx43 reflect the negative relationship ,which shows that microRNA cannot participate in the atrial structural remodeling through regulat-ing the expression of Cx43 directly.
