中外健康文摘
中外健康文摘
중외건강문적
WORLD HEALTH DIGEST
2013年
41期
31-32
,共2页
虎杖水煎液%衰老%学习%记忆%Y迷宫%小鼠
虎杖水煎液%衰老%學習%記憶%Y迷宮%小鼠
호장수전액%쇠로%학습%기억%Y미궁%소서
Rhizoma%Polygoni%Cuspidati decoction aging learning memory%Y-maze mouse
目的：观察虎杖水煎液对D-半乳糖（D-g a l）所致衰老小鼠学习、记忆功能的影响，并初步探讨其作用机制。方法取I C R小鼠80只，雌雄兼用，随机分成4组：正常对照组（NG，n=20），模型对照组（MG，n=20），虎杖水煎液低剂量组（LG，5g/kg，n=20），虎杖水煎液高剂量组（HG，10g/kg， n=20）。正常对照组给予背部皮下注射生理盐水，其余各组用 D-gal按150mg/kg背部皮下注射6周，每日一次，造成亚急性衰老模型。造模同时，虎杖水煎液低剂量组和高剂量组分别给予相应浓度的虎杖水煎液，正常对照组和模型对照组给予相同容积的0.5%羧甲基纤维素钠（CMC）。采用Y型电迷宫检测学习、记忆功能；使用相关试剂盒测定脑皮层中超氧化物歧化酶（SOD）和单胺氧化酶-B（MAO-B）的活力及丙二醛（MDA）和脂褐质（LP）的含量。结果给予D-gal 6周后，Y迷宫检测模型组记忆功能明显下降，表现为不同时间的正确反应率降低，潜伏期延长；而虎杖水煎液能明显缩短衰老小鼠潜伏期，显著升高正确反应率。生化检测指标显示，模型组脑皮层中MDA和LP含量升高；虎杖水煎液能降低脑皮层中MAO-B活性，升高SOD活性，降低脑皮层中MDA和LP含量。结论虎杖水煎液对D-gal诱导衰老模型小鼠的记忆功能有保护作用，其机制可能与其减少脑中MDA及LP含量，降低MAO-B活性，升高SOD活性有关。
目的：觀察虎杖水煎液對D-半乳糖（D-g a l）所緻衰老小鼠學習、記憶功能的影響，併初步探討其作用機製。方法取I C R小鼠80隻，雌雄兼用，隨機分成4組：正常對照組（NG，n=20），模型對照組（MG，n=20），虎杖水煎液低劑量組（LG，5g/kg，n=20），虎杖水煎液高劑量組（HG，10g/kg， n=20）。正常對照組給予揹部皮下註射生理鹽水，其餘各組用 D-gal按150mg/kg揹部皮下註射6週，每日一次，造成亞急性衰老模型。造模同時，虎杖水煎液低劑量組和高劑量組分彆給予相應濃度的虎杖水煎液，正常對照組和模型對照組給予相同容積的0.5%羧甲基纖維素鈉（CMC）。採用Y型電迷宮檢測學習、記憶功能；使用相關試劑盒測定腦皮層中超氧化物歧化酶（SOD）和單胺氧化酶-B（MAO-B）的活力及丙二醛（MDA）和脂褐質（LP）的含量。結果給予D-gal 6週後，Y迷宮檢測模型組記憶功能明顯下降，錶現為不同時間的正確反應率降低，潛伏期延長；而虎杖水煎液能明顯縮短衰老小鼠潛伏期，顯著升高正確反應率。生化檢測指標顯示，模型組腦皮層中MDA和LP含量升高；虎杖水煎液能降低腦皮層中MAO-B活性，升高SOD活性，降低腦皮層中MDA和LP含量。結論虎杖水煎液對D-gal誘導衰老模型小鼠的記憶功能有保護作用，其機製可能與其減少腦中MDA及LP含量，降低MAO-B活性，升高SOD活性有關。
목적：관찰호장수전액대D-반유당（D-g a l）소치쇠로소서학습、기억공능적영향，병초보탐토기작용궤제。방법취I C R소서80지，자웅겸용，수궤분성4조：정상대조조（NG，n=20），모형대조조（MG，n=20），호장수전액저제량조（LG，5g/kg，n=20），호장수전액고제량조（HG，10g/kg， n=20）。정상대조조급여배부피하주사생리염수，기여각조용 D-gal안150mg/kg배부피하주사6주，매일일차，조성아급성쇠로모형。조모동시，호장수전액저제량조화고제량조분별급여상응농도적호장수전액，정상대조조화모형대조조급여상동용적적0.5%최갑기섬유소납（CMC）。채용Y형전미궁검측학습、기억공능；사용상관시제합측정뇌피층중초양화물기화매（SOD）화단알양화매-B（MAO-B）적활력급병이철（MDA）화지갈질（LP）적함량。결과급여D-gal 6주후，Y미궁검측모형조기억공능명현하강，표현위불동시간적정학반응솔강저，잠복기연장；이호장수전액능명현축단쇠로소서잠복기，현저승고정학반응솔。생화검측지표현시，모형조뇌피층중MDA화LP함량승고；호장수전액능강저뇌피층중MAO-B활성，승고SOD활성，강저뇌피층중MDA화LP함량。결론호장수전액대D-gal유도쇠로모형소서적기억공능유보호작용，기궤제가능여기감소뇌중MDA급LP함량，강저MAO-B활성，승고SOD활성유관。
Aim: To investigate the effect of Rhizoma Polygoni Cuspidati (RPC) decoction on learning and memory function of aging model mice induced by D-galactose, and explore its mechanism preliminarily. Methods: 80 ICR mice, male and female were both used, were randomly divided into 4 groups: Normal control group (NG, n=20), Model control group (MG, n=20), Low-dose drug treatment group (LG,5g/kg,n=20), High-dose drug treatment group (HG,10g/kg,n=20).Except NG was subeutaneously injected with saline, other groups were given subcutaneously injection of D-galactose (D-gal) at 150 mg/kg once a day for six weeks to induce subacute aging model. At the same time LG was oral y administrated RPC decoction at dose 5g/kg and HG at 10g/kg. NG and MG were given oral y the same volume 0.5% carboxymethyl cellulose sodium(CMC). Y-maze was use to determine the function of learning and memory. The analytic kit was used to measure the content of malondialdehyde (MDA) and lipofuscin (LP), the activity of superoxide dismutase(SOD) and monoamine oxidase-B (MAO-B) in brain. Results: In Y-maze test, the correct response rate declined and the latency of first correct response has extended in MG. RPC decoction could obviously reduce the latency of first correct response and the frequency which arrived the standard of continual three right response in aging model mice with significant difference and elevate remarkably correct response rate of model mice. The results of biochemical detection indicated, the content of LP and MDA in brain increased in MG, RPC decoction could decrease MAO-B activity and content of LP and MDA , increase SOD activity in brain in aging model mice. Conclusions: RPC decoction has protective effect on memory in aging model mice produced by D-galactose. Its mechanism may be relate to its action of reducing MAO-B activity, decreasing LP and MDA contents, increasing SOD activity in brain.
